Dietary resveratrol prevents the development of food allergy in mice.
Dietary resveratrol prevents the development of food allergy in mice.
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DOI：
10.1371/journal.pone.0044338
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Nakao A
中科院分区：
文献类型：
--
作者：
Okada Y;Oh-oka K;Nakamura Y;Ishimaru K;Matsuoka S;Okumura K;Ogawa H;Hisamoto M;Okuda T;Nakao A
白藜芦醇是一种富含于红葡萄酒中的生物活性多酚，通过多种机制表现出许多有益健康的作用。然而，目前还不清楚白藜芦醇是否有利于预防食物过敏。本研究通过小鼠食物过敏模型研究白藜芦醇是否能抑制食物过敏的发生。用卵白蛋白（OVA）和霍乱毒素（CT）粘膜佐剂灌胃致敏饲喂标准饲料或标准饲料加白藜芦醇的小鼠。然后在小鼠之间比较食物过敏的几种表现。白藜芦醇对T细胞或树突状细胞的影响也通过使用来自OVA特异性T细胞受体（TCR）转基因DO11.10小鼠的脾细胞或小鼠骨髓来源的树突状细胞（BMDCs）在体外进行了检查。我们发现，与对照小鼠相比，在口服OVA加CT致敏后，喂食白藜芦醇的小鼠显示出OVA特异性血清IgE产生减少，过敏反应以及OVA诱导的肠系膜淋巴结（MLN）和脾脏IL-13和IFN-α产生减少。此外，白藜芦醇抑制OVA加CT诱导的DO11.10小鼠脾细胞中IL-4、IL-13和IFN-α的产生，并抑制加塔-3和T-bet表达。此外，白藜芦醇抑制OVA加CT诱导的DO11.10小鼠脾细胞中CD 25表达和IL-2产生，并降低CD 80和CD 86表达水平。最后，白藜芦醇抑制CT诱导的cAMP升高与BMDCs中CD 80和CD 86表达水平的降低相关。白藜芦醇的摄入防止了小鼠食物过敏模型的发展。鉴于体外研究结果，白藜芦醇可能通过抑制小鼠中CT诱导的cAMP活化来抑制DC成熟和随后的早期T细胞活化和分化。这些结果表明，白藜芦醇可能具有预防食物过敏的潜力。
Resveratrol is a bioactive polyphenol enriched in red wine that exhibits many beneficial health effects via multiple mechanisms. However, it is unclear whether resveratrol is beneficial for the prevention of food allergy. This study investigated whether resveratrol inhibited the development of food allergy by using a mouse model of the disease. Mice fed standard diet or standard diet plus resveratrol were sensitized by intragastric administration of ovalbumin (OVA) and mucosal adjuvant cholera toxin (CT). Several manifestations of food allergy were then compared between the mice. The effects of resveratrol on T cells or dendritic cells were also examined by using splenocytes from OVA-specific T cell-receptor (TCR) transgenic DO11.10 mice or mouse bone marrow-derived dendritic cells (BMDCs) in vitro. We found that mice fed resveratrol showed reduced OVA-specific serum IgE production, anaphylactic reaction, and OVA-induced IL-13 and IFN-ã production from the mesenteric lymph nodes (MLNs) and spleens in comparison to the control mice, following oral sensitization with OVA plus CT. In addition, resveratrol inhibited OVA plus CT-induced IL-4, IL-13, and IFN-ã production in splenocytes from DO11.10 mice associated with inhibition of GATA-3 and T-bet expression. Furthermore, resveratrol suppressed the OVA plus CT-induced CD25 expression and IL-2 production in DO11.10 mice-splenocytes in association with decreases in CD80 and CD86 expression levels. Finally, resveratrol suppressed CT-induced cAMP elevation in association with decreases in CD80 and CD86 expression levels in BMDCs. Ingestion of resveratrol prevented the development of a food allergy model in mice. Given the in vitro findings, resveratrol might do so by inhibiting DC maturation and subsequent early T cell activation and differentiation via downregulation of CT-induced cAMP activation in mice. These results suggest that resveratrol may have potential for prophylaxis against food allergy.
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影响因子：
8.7
作者：
Weiner HL;da Cunha AP;Quintana F;Wu H
通讯作者：
Wu H
DOI：
10.1111/j.1749-6632.2010.05853.x
发表时间：
2011-01-01
期刊：
RESVERATROL AND HEALTH
影响因子：
--
作者：
Patel, Ketan R.;Scott, Edwina;Brown, Karen
通讯作者：
Brown, Karen
影响因子：
64.8
作者：
Baur, Joseph A.;Pearson, Kevin J.;Sinclair, David A.
通讯作者：
Sinclair, David A.
影响因子：
4.4
作者：
Blazquez, Ana Belen;Berin, M. Cecilia
通讯作者：
Berin, M. Cecilia
影响因子：
4.6
作者：
Sharma, S.;Chopra, K.;Agrewala, J. N.
通讯作者：
Agrewala, J. N.