Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.
Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.
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膜联蛋白A2和人附睾蛋白4在子宫内膜癌中的表达及临床意义
DOI：
10.1186/s13046-015-0208-8
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发表时间：
2015-09-11
期刊：
影响因子：
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通讯作者：
Lin B
中科院分区：
文献类型：
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作者：
Deng L;Gao Y;Li X;Cai M;Wang H;Zhuang H;Tan M;Liu S;Hao Y;Lin B
背景众所周知，晚期子宫内膜癌的治疗和监测手段有限。在肿瘤进展过程中表达变化的生物分子成为晚期子宫内膜癌潜在的治疗靶点。膜联蛋白A2(Annexin A2，ANXA2)在复发性子宫内膜癌中过表达，人附睾蛋白4(HE4)在子宫内膜癌中表达上调。此外，ANXA2和HE4在卵巢癌中相互作用，促进卵巢癌的恶性生物学行为。我们推测它们之间的相互作用可能也存在于子宫内膜癌中。方法采用免疫组织化学方法检测84例子宫内膜癌、30例子宫内膜不典型增生和18例正常子宫内膜组织中ANXA2和HE4蛋白的表达。结果ANXA2和HE4共定位于子宫内膜组织和子宫内膜癌细胞中。ANXA2和HE4在子宫内膜癌中的阳性表达率分别为95.2%和85.7%，显著高于正常子宫内膜(55.6%和16.7%，P<0.05和 <0.05)。ANXA2和HE4的表达与FIGO分期、分化程度、肌层侵袭和淋巴结转移显著相关。ANXA2是影响子宫内膜癌预后的独立危险因素(P<； 为8.004，危险性比[HR] = 为8.004)。ANXA2和HE4的表达呈正相关(Spearman相关系数 = 为0.228，p<； 为0.05)。在多元线性回归模型中，HE4是ANXA2的独立影响因素(p<； )。ANXA2和HE4的表达水平与子宫内膜癌的恶性生物学行为密切相关，ANXA2是预后不良的独立危险因素。ANXA2和HE4的表达可以相互影响。
BackgroundIt is well-known that the treatment and monitoring methods are limited for advanced stage of endometrial carcinoma. Biological molecules with expression changes during tumor progression become potential therapeutic targets for advanced stage endometrial carcinoma. Annexin A2 (ANXA2) has been reported to be overexpressed in recurrent endometrial carcinoma, and the expression of human epididymis protein 4 (HE4) is upregulated in endometrial carcinoma. What’s more, ANXA2 and HE4 interacted in ovarian cancer and promoted the malignant biological behavior. We speculated that their interaction may exist in endometrial carcinoma as well. We evaluated the expression and the correlation relationship of ANXA2 and HE4 in endometrial carcinoma.MethodsThe expression of ANXA2 and HE4 protein in 84 endometrial carcinoma, 30 endometrial atypical hyperplasia, and 18 normal endometrial tissue samples were then measured using an immunohistochemical assay in paraffin embedded endometrial tissues. The structural relationship between ANXA2 and HE4 was explored by immunoprecipitation and double immunofluorescent staining.ResultsANXA2 and HE4 co-localized in both endometrial tissues and endometrial carcinoma cells. ANXA2 and HE4 were expressed in 95.2 % and 85.7 % of the the endometrial carcinoma, respectively, which were significantly higher than normal endometrium (55.6 % and 16.7 %, bothp< 0.05). The expression of ANXA2 and HE4 was significantly correlated with FIGO stage, degree of differentiation, myometrial invasion, and lymph node metastasis. ANXA2 was an independent risk factor for the prognosis of endometrial carcinoma (p< 0.05, hazard ratio [HR] = 8.004). The expression of ANXA2 and HE4 was positively correlated (Spearman correlation coefficient = 0.228,p< 0.05). HE4 was an independent factor for ANXA2 in multivariate linear regression model (p< 0.05).ConclusionWe revealed the co-localization of ANXA2 and HE4 in endometrial carcinoma. Expression levels of ANXA2 and HE4 were closely related to the malignant biological behavior of endometrial carcinoma, and ANXA2 was an independent risk factor for poor prognosis. The expression of ANXA2 and HE4 can affect each other.
DOI：
10.1186/1756-9966-27-64
发表时间：
2008-11-05
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
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作者：
Miszczak-Zaborska E;Kubiak R;Bieńkiewicz A;Bartkowiak J
通讯作者：
Bartkowiak J