The cytosol activity of thymidine phosphorylase in endometrial cancer.
The cytosol activity of thymidine phosphorylase in endometrial cancer.
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DOI：
10.1186/1756-9966-27-64
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发表时间：
2008-11-05
期刊：
影响因子：
--
通讯作者：
Bartkowiak J
中科院分区：
文献类型：
--
作者：
Miszczak-Zaborska E;Kubiak R;Bieńkiewicz A;Bartkowiak J
胸苷磷酸化酶（TP）与促进血管生成的血小板源性内皮细胞生长因子（PD-ECGF）相同。本研究的目的是评估子宫内膜癌患者肿瘤样本中TP的细胞质活性。用分光光度法测定43例子宫内膜肿瘤细胞质中TP的活性。采用免疫组化染色法检测血小板源性内皮细胞生长因子/胸苷磷酸化酶（PD-ECGF/TP）蛋白表达及微血管密度（MD）。16名因非肿瘤原因接受手术治疗的女性的正常子宫内膜作为对照。探讨胞浆TP活性、PD-ECGF/TP蛋白表达、MD与临床病理特征的关系。与对照组（正常子宫内膜样本）相比，恶性肿瘤样本的胞浆TP活性、PD-ECGF/TP蛋白表达和MD显著升高。胞质酶活性与PD-ECGF/TP蛋白表达与MD呈显著正相关，其余与PD-ECGF/TP蛋白表达与MD相关性较弱。此外，细胞质TP活性、PD-ECGF/TP蛋白表达、MD与子宫内膜癌分级或组织学类型均无相关性。子宫内膜癌的胞浆TP活性明显高于正常子宫内膜，与肿瘤的分期和分级无关，但与PD-ECGF/TP蛋白表达和MD相关，因此可能与术后化疗或放疗患者预后良好有关。
Thymidine phosphorylase (TP) is identical with platelet-derived endothelial cell growth factor (PD-ECGF) which promotes angiogenesis. The aim of this study was to evaluate the cytosol activity of TP in tumor samples from patients with endometrial cancer. The activity of TP was measured by the spectrophotometric method in the cytosol of endometrial tumor samples from 43 patients. Moreover, the expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) protein and microvessel density (MD) were examined in the same endometrial tumor samples by immunohistochemical staining. Normal endometrium from 16 women, treated surgically due to nononcological reasons served as a control. A relationship between the cytosol TP activity, PD-ECGF/TP protein expression, MD and clinicopathologic features was investigated. A significantly higher the cytosol TP activity, PD-ECGF/TP protein expression and MD was stated in malignant tumor samples when compared to the control (samples of normal endometrium). A positive statistically significant correlation between the cytosol enzyme activity and PD-ECGF/TP protein expression and MD was found, but weaker from the remaining ones between PD-ECGF/TP protein expression and MD was observed. Besides no correlation between the cytosol TP activity, PD-ECGF/TP protein expression as well as MD and grading or histopatological type of endometrial cancer was stated. The cytosol TP activity in endometrial cancer is significantly higher than in normal endometrium, with no relation as to the stage and grade of tumors, but correlates with the PD-ECGF/TP protein expression and MD may therefore be associated with favorable prognosis in patients treated with chemo- or radiotherapy after surgery.
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影响因子：
2.4
作者：
Saeki, T;Tanada, M;Moriwaki, S
通讯作者：
Moriwaki, S
影响因子：
2.9
作者：
MOGHADDAM, A;BICKNELL, R
通讯作者：
BICKNELL, R
影响因子：
1.3
作者：
Bijnsdorp, I. V.;de Bruin, M.;Peters, G. J.
通讯作者：
Peters, G. J.
胸苷磷酸化酶/血小板衍生的内皮细胞生长因子表达与胃癌中的肝转移相关。
影响因子：
8.8
作者：
Maeda, K;Chung, Y S;Ogawa, Y;Takatsuka, S;Kang, S M;Ogawa, M;Sawada, T;Onoda, N;Kato, Y;Sowa, M
通讯作者：
Sowa, M
影响因子：
7.2
作者：
ABULAFIA, O;TRIEST, WE;GHEZZI, F
通讯作者：
GHEZZI, F