Substrate-Mediated Laser Ablation under Ambient Conditions for Spatially-Resolved Tissue Proteomics
Substrate-Mediated Laser Ablation under Ambient Conditions for Spatially-Resolved Tissue Proteomics
复制标题
环境条件下基质介导的激光烧蚀用于空间分辨组织蛋白质组学
作者：
B. Fatou;M. Wisztorski;C. Focsa;M. Salzet;M. Ziskind;I. Fournier
在过去的十年中，环境质谱法（MS）的许多应用已经得到证实。他们促进了各种微取样技术的出现，如激光烧蚀/液滴捕获（LADC）。LADC包括从表面烧蚀分析物以及随后将其捕获在溶剂液滴中，然后可以通过MS进行分析。因此，LADC通常在UV或IR范围内使用分析物或基质吸收的波长进行。在这项工作中，我们探讨了潜在的可见光范围LADC（532 nm）作为一种微取样技术，用于大规模的蛋白质组学分析。我们证明，使用532 nm LADC的生物分子分析是可能的，尽管在这个波长的生物分子的吸光度低。这是由于在低激光能量下间接衬底介导的烧蚀机制的优势，这与由样品吸收驱动的常规直接烧蚀形成对比。使用我们的定制LADC系统并利用这种底物介导的消融机制，我们能够对微量取样组织切片进行大规模蛋白质组学分析，并证明了可能鉴定具有相关生物学功能的蛋白质。因此，532 nm LADC技术为生物学和临床应用提供了一种新的工具。
Numerous applications of ambient Mass Spectrometry (MS) have been demonstrated over the past decade. They promoted the emergence of various micro-sampling techniques such as Laser Ablation/Droplet Capture (LADC). LADC consists in the ablation of analytes from a surface and their subsequent capture in a solvent droplet which can then be analyzed by MS. LADC is thus generally performed in the UV or IR range, using a wavelength at which analytes or the matrix absorb. In this work, we explore the potential of visible range LADC (532 nm) as a micro-sampling technology for large-scale proteomics analyses. We demonstrate that biomolecule analyses using 532 nm LADC are possible, despite the low absorbance of biomolecules at this wavelength. This is due to the preponderance of an indirect substrate-mediated ablation mechanism at low laser energy which contrasts with the conventional direct ablation driven by sample absorption. Using our custom LADC system and taking advantage of this substrate-mediated ablation mechanism, we were able to perform large-scale proteomic analyses of micro-sampled tissue sections and demonstrated the possible identification of proteins with relevant biological functions. Consequently, the 532 nm LADC technique offers a new tool for biological and clinical applications.
使用激光诱导声解吸/化学电离、大气压化学电离和电喷雾电离质谱表征非极性脂质和选定的类固醇。
影响因子：
1.8
作者：
Jin,Zhicheng;Daiya,Shivani;Kenttämaa,HilkkaI
通讯作者：
Kenttämaa,HilkkaI
通过脉冲激光烧蚀冷冻水溶液来挥发高分子量 DNA。
DOI：
10.1126/science.2595370
发表时间：
1989
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Nelson,RW;Rainbow,MJ;Lohr,DE;Williams,P
通讯作者：
Williams,P
影响因子：
6.6
作者：
Wu, Chunping;Dill, Allison L.;Eberlin, Livia S.;Cooks, R. Graham;Ifa, Demian R.
通讯作者：
Ifa, Demian R.