Ontogenetic changes in cutaneous and branchial ionocytes and morphology in yellowfin tuna (Thunnus albacares) larvae
Ontogenetic changes in cutaneous and branchial ionocytes and morphology in yellowfin tuna (Thunnus albacares) larvae
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黄鳍金枪鱼（Thunnus albacares）幼鱼皮肤和鳃离子细胞及形态的个体发生变化
DOI：
10.1007/s00360-018-1187-9
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
M. Tresguerres
中科院分区：
文献类型：
--
作者：
G. Kwan;J. Wexler;N. Wegner;M. Tresguerres
研究了黄鳍金枪鱼幼鱼（Thunnus albacares）在孵化后2 ~ 25天（2.9 ~ 24.5 mm标准长度，SL）渗透调节器官和气体交换器官的发育情况。采用Na+/K+- atp酶免疫染色和扫描电镜对皮肤和鳃离子细胞进行鉴定。皮肤离子细胞丰度随SL显著增加，但离子细胞大小和密度的减少导致相对离子细胞面积显著减少。预屈曲幼虫表皮离子细胞顶端开口较宽，微绒毛较长；然而，微绒毛从屈曲开始缩回到尖窝。在鳃和假分支中，在约3.3 mm SL中首先检测到片层。离子细胞一直存在于鳃弓上，在3.4 mm SL中首先出现在假分支的丝和片层中，后来在4.2 mm SL中出现在鳃丝中，但在鳃片中未观察到离子细胞。与皮离子细胞不同，鳃和假分支离子细胞在整个幼虫发育过程中具有宽的顶端开口和延伸的微绒毛。板层间融合是一种特殊的鳃结构，结合了ram通气鱼的板层，在约24.5 mm SL开始形成，并含有离子细胞，这是以前从未报道过的定位。在较大的亚成年黄鳍金枪鱼的片层融合体和细丝融合体上也发现了离子细胞；然而，亚成体鳃离子细胞有顶凹。这些结果表明，在弯曲期，气体交换和NaCl分泌从皮肤转移到鳃器官，并揭示了在挤压通气鱼类中离子细胞定位和形态的新方面。
The development of osmoregulatory and gas exchange organs was studied in larval yellowfin tuna (Thunnus albacares) from 2 to 25 days post-hatching (2.9–24.5 mm standard length, SL). Cutaneous and branchial ionocytes were identified using Na+/K+-ATPase immunostaining and scanning electron microscopy. Cutaneous ionocyte abundance significantly increased with SL, but a reduction in ionocyte size and density resulted in a significant decrease in relative ionocyte area. Cutaneous ionocytes in preflexion larvae had a wide apical opening with extended microvilli; however, microvilli retracted into an apical pit from flexion onward. Lamellae in the gill and pseudobranch were first detected ~ 3.3 mm SL. Ionocytes were always present on the gill arch, first appeared in the filaments and lamellae of the pseudobranch at 3.4 mm SL, and later in gill filaments at 4.2 mm SL, but were never observed in the gill lamellae. Unlike the cutaneous ionocytes, gill and pseudobranch ionocytes had a wide apical opening with extended microvilli throughout larval development. The interlamellar fusion, a specialized gill structure binding the lamellae of ram-ventilating fish, began forming by ~ 24.5 mm SL and contained ionocytes, a localization never before reported. Ionocytes were retained on the lamellar fusions and also found on the filament fusions of larger sub-adult yellowfin tuna; however, sub-adult gill ionocytes had apical pits. These results indicate a shift in gas exchange and NaCl secretion from the skin to branchial organs around the flexion stage, and reveal novel aspects of ionocyte localization and morphology in ram-ventilating fishes.
氯细胞和渗透压调节。
影响因子：
19.6
作者：
Zadunaisky,JA
通讯作者：
Zadunaisky,JA