Anti-allergic and anti-inflammatory effects of resveratrol via inhibiting TXNIP-oxidative stress pathway in a mouse model of allergic rhinitis.
Anti-allergic and anti-inflammatory effects of resveratrol via inhibiting TXNIP-oxidative stress pathway in a mouse model of allergic rhinitis.
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白藜芦醇通过抑制TXNIP氧化应激通路对变应性鼻炎小鼠模型的抗过敏和抗炎作用
DOI：
10.1016/j.waojou.2020.100473
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Lin H
中科院分区：
文献类型：
--
作者：
Zhang W;Tang R;Ba G;Li M;Lin H
变应性鼻炎（AR）是一种由鼻内IgE介导的I型超敏反应。硫氧还蛋白相互作用蛋白（TXNIP）在活性氧（ROS）产生过程中起着关键作用。白藜芦醇是一种TXNIP抑制剂。然而，其在AR中的作用和机制仍然不确定。本研究旨在探讨白藜芦醇对卵清蛋白（OVA）诱导的小鼠AR模型的作用及其机制。用卵清蛋白（OVA）建立AR小鼠模型，分别鼻内给予白藜芦醇（resveratrol）和N-乙酰半胱氨酸（NAC）。苏木精-伊红（HE）染色用于评价嗜酸性粒细胞。采用免疫组织化学（IHC）染色和实时荧光定量PCR（real-time PCR）方法检测小鼠鼻粘膜中TXNIP的免疫标记和mRNA表达。采用丙二醛（MDA）和超氧化物歧化酶（SOD）测定试剂盒测定鼻组织匀浆中丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性。ELISA法检测血清中OVA特异性IgE、组胺、鼻灌洗液（NLF）中OVA特异性IgE、PGD 2、LTC 4、ECP、IL-4、IL-5、IL-6、IL-33和TNF-α的浓度。采用Western blotting、real-time PCR、ELISA、DCFH-DA、MDA和SOD检测试剂盒等方法，研究了OVA、白藜芦醇和NAC对脾单个核细胞的作用及其机制。我们发现白藜芦醇或NAC治疗的小鼠与未治疗的AR小鼠相比，打喷嚏，擦鼻，炎性细胞因子，嗜酸性粒细胞数量，TXNIP，MDA和SOD水平的显着变化。在培养的脾单个核细胞中，TXNIP、MDA、SOD、ROS和炎性细胞因子水平被OVA改变，但被白藜芦醇或NAC逆转。白藜芦醇可通过抑制TXNIP-氧化应激途径有效缓解AR。
Allergic rhinitis (AR) is a type I hypersensitivity mediated by IgE in the nose. Thioredoxin-interacting protein (TXNIP) plays a pivotal role in the process of producing reactive oxygen species (ROS). Resveratrol is a TXNIP inhibitor. Nonetheless, its role and mechanism in AR are still undetermined. The present study aimed to explore the effect and mechanism of resveratrol on an ovalbumin (OVA) induced mouse model of AR. AR murine model was established using OVA and administrated intranasally with resveratrol or N-acetylcysteine (NAC). Hematoxylin and eosin (HE) stain was used for evaluating eosinophils. Immunohistochemistry (IHC) staining and real-time PCR were employed to evaluate immunolabeling and mRNA expression of TXNIP in nasal mucosas of mice. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in nasal tissue homogenates were measured using MDA and SOD Assay Kit. Concentrations of OVA-specific IgE and histamines in serum, and OVA-specific IgE, PGD2, LTC4, ECP, IL-4, IL-5, IL-6, IL-33 and TNF-α in nasal lavage fluid (NLF) were assayed by ELISA. In vitro studies, western blotting, real-time PCR, ELISA, ROS detecting dye DCFH-DA, MDA, and SOD Assay Kit were performed to evaluate the effects and mechanisms of OVA, resveratrol or NAC on spleen mononuclear cells. We found significant alternations of sneezing, nasal rubbing, inflammatory cytokines, eosinophil numbers, TXNIP, MDA, and SOD levels in resveratrol or NAC treated mice compared with untreated AR mice. In cultured spleen mononuclear cells, TXNIP, MDA, SOD, ROS and inflammatory cytokines levels were altered by OVA but reversed by resveratrol or NAC. Resveratrol could effectively alleviate murine AR by inhibiting TXNIP-oxidative stress pathway.
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DOI：
10.1093/gerona/glv071
发表时间：
2016-06-01
影响因子：
5.1
作者：
Bedarida, Tatiana;Baron, Stephanie;Nivet-Antoine, Valerie
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DOI：
10.1111/j.1365-2222.2010.03549.x
发表时间：
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期刊：
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
影响因子：
--
作者：
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