Improved survival of mesenchymal stromal cell after hypoxia preconditioning: role of oxidative stress.
Improved survival of mesenchymal stromal cell after hypoxia preconditioning: role of oxidative stress.
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DOI：
10.1016/j.lfs.2010.10.023
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发表时间：
2011-01-03
期刊：
影响因子：
6.1
通讯作者：
Rodriguez-Porcel, Martin
中科院分区：
文献类型：
--
作者：
Peterson, Karen M.;Aly, Abdelrahman;Lerman, Amir;Lerman, Lilach O.;Rodriguez-Porcel, Martin
研究低氧预处理 (HPC) 对间充质基质细胞 (MSC) 有益作用的机制，并优化新型非侵入性方法来评估旨在提高细胞存活率的生物干预措施的效果。来自大鼠股骨的 MSC，无论有或没有 HPC，均暴露于细胞培养物的低氧条件下（1% O2，持续 24 小时），并测量细胞存活率（通过 LDH 释放测定和膜联蛋白-V 染色）。对氧化状态（二氯荧光素-DCF-和二氢乙锭-DHE-的转化、氧化酶的蛋白质表达）以及应激下细胞的迁移模式进行了表征。此外，使用最先进的分子成像非侵入性地评估细胞存活率。与对照组相比，缺氧导致氧化应激酶 NAD(P)H 氧化酶（亚基 67phox：分别为 0.05 ± 0.01 AU 和 0.48 ± 0.02 AU，p<0.05）和 ROS 量（DCF：分别为 13 ± 1 和 42 ± 3 RFU/μg 蛋白，p<0.05）的表达增加，从而导致 ROS 含量下降 干细胞活力。缺氧预处理保留了细胞生物学，如氧化状态（16 ± 1 RFU/μg 蛋白质，p<0.05 与缺氧相比）和细胞活力的保留所证明。最重要的是，可以使用分子成像非侵入性地评估 HPC 的有益效果。 HPC 部分通过保存氧化剂状态来保留细胞活力和功能，并且可以使用最先进的分子成像来评估其效果。了解干细胞命运的机制对于干细胞治疗领域的进步至关重要。
To investigate the mechanisms underlying the beneficial effect of hypoxia preconditioning (HPC) on mesenchymal stromal cells (MSCs) and optimize novel non-invasive methods to assess the effect of biological interventions aimed to increased cell survival. MSCs from rat femur, with or without HPC, were exposed to hypoxic conditions in cell culture (1% O2 for 24 h) and cell survival (by the LDH release assay and Annexin-V staining) was measured. Oxidant status (conversion of dichloro-fluorescein-DCF- and dihydro-ethidium-DHE-, protein expression of oxidant enzymes) was characterized, together with the mobility pattern of cells under stress. Furthermore, cell survival was assessed non-invasively using state-of-the-art molecular imaging. Compared to controls, Hypoxia resulted in increased expression of the oxidative stress enzyme NAD(P)H oxidase (subunit 67phox: 0.05 ± 0.01 AU and 0.48 ± 0.02 AU, respectively, p<0.05) and in the amount of ROS (DCF: 13 ± 1 and 42 ± 3 RFU/µg protein, respectively, p<0.05) which led to a decrease in stem cell viability. Hypoxia preconditioning preserved cell biology, as evidenced by preservation of oxidant status (16 ± 1 RFU/µg protein, p<0.05 vs. hypoxia), and cell viability. Most importantly, the beneficial effect of HPC can be assessed non-invasively using molecular imaging. HPC preserves cell viability and function, in part through preservation of oxidant status, and its effects can be assessed using state-of-the-art molecular imaging. Understanding of the mechanisms underlying the fate of stem cells will be critical for the advancement of the field of stem cell therapy.
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影响因子：
3.7
作者：
Geft, Dael;Schwartzenberg, Shmuel;Rogowsky, Ori;Finkelstein, Ariel;Ablin, Jacob;Maysel-Auslender, Sofia;Wexler, Dov;Keren, Gad;George, Jacob
通讯作者：
George, Jacob
影响因子：
3.3
作者：
Ksiazek, K;Piwocka, K;Witowski, J
通讯作者：
Witowski, J
影响因子：
5.6
作者：
Kanichai, Manoj;Ferguson, Damien;Campbell, Veronica A.
通讯作者：
Campbell, Veronica A.
影响因子：
2.8
作者：
Lim, SD;Sun, C;Arnold, RS
通讯作者：
Arnold, RS
DOI：
10.1016/j.ijrobp.2003.10.022
发表时间：
2004-03-01
影响因子：
7
作者：
Epperly, MW;Osipov, AN;Kagan, VE
通讯作者：
Kagan, VE