Circulating apoptotic progenitor cells in patients with congestive heart failure.
Circulating apoptotic progenitor cells in patients with congestive heart failure.
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DOI：
10.1371/journal.pone.0003238
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发表时间：
2008-09-18
期刊：
影响因子：
3.7
通讯作者：
George, Jacob
中科院分区：
文献类型：
--
作者：
Geft, Dael;Schwartzenberg, Shmuel;Rogowsky, Ori;Finkelstein, Ariel;Ablin, Jacob;Maysel-Auslender, Sofia;Wexler, Dov;Keren, Gad;George, Jacob
循环中的CD 34+内皮祖细胞（EPCs）能够分化为成熟的内皮细胞，以帮助血管生成和血管生成。我们试图量化充血性心力衰竭患者中凋亡祖细胞的数量。采用Ficoll密度梯度离心法分离58例不同程度心力衰竭患者和23例对照者外周血单个核细胞。使用膜联蛋白V-PE/PI检测试剂盒评估祖细胞CD 34+细胞中的凋亡，并使用CD 34、膜联蛋白-V和碘化丙啶的三重染色进行FACS分析。在受试者组中测定早期和晚期凋亡祖细胞的百分比，并与临床特征相关。虽然对照组和CHF患者之间（p = 0.42）或重度和轻度/中度CHF组之间（p = 0.544）的总CD 34阳性细胞或早期凋亡祖细胞没有显著差异，但重度CHF组中晚期凋亡祖细胞的数量高于轻度/中度CHF组（p = 0.03）。 与匹配的对照组相比，CHF患者中晚期凋亡祖细胞显著增加。晚期凋亡祖细胞与射血分数之间也存在负相关（r =-0.252，p = 0.028），与NYHA分级呈正相关（r = 0.223，p = 0.046）。 重度心力衰竭患者晚期凋亡祖细胞数量较多，与NYHA分级呈正相关，与射血分数呈负相关。这一发现可能揭示了许多因素的CHF的病理生理。
Circulating CD34+ endothelial progenitor cells (EPCs) are capable of differentiating into mature endothelial cells to assist in angiogenesis and vasculogenesis. We sought to quantify the numbers of apoptotic progenitors in patients with congestive heart failure. Peripheral blood mononuclear cells were isolated by Ficoll density-gradient from 58 patients with various degrees of heart failure and 23 matched controls. Apoptosis in progenitor CD34+ cells was assessed using the Annexin V-PE/PI detection kit, and FACS analysis was performed with triple staining for CD34, annexin-V and propidium iodide. The percentage of early and late apoptotic progenitor cells was determined in the subject groups and was correlated with clinical characteristics. While there was no significant difference in total CD34 positive cells or early apoptotic progenitors between control subjects and CHF patients (p = 0.42) or between severe and mild/moderate CHF groups (p = 0.544), there was an elevated number of late apoptotic progenitors in the severe CHF group compared with the mild/moderate CHF group (p = 0.03). Late apoptotic progenitors were significantly increased in CHF patients as compared to matched controls. There was also an inverse correlation between late apoptotic progenitors and ejection fraction (r = −0.252, p = 0.028) as well as a positive association with NYHA class (r = 0.223, p = 0.046). Severe heart failure patients exhibited higher numbers of late apoptotic progenitors, and this was positively associated with NYHA class and negatively correlated with ejection fraction. This finding may shed light on the numerous factors governing the pathophysiology of CHF.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
18.2
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2.9
作者：
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1.1
作者：
Chin, BSP;Conway, DSG;Lip, GYH
通讯作者：
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