Differential proteomic analysis by iTRAQ reveals the growth mechanism in Pyropia yezoensis mutant
Differential proteomic analysis by iTRAQ reveals the growth mechanism in Pyropia yezoensis mutant
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iTRAQ 差异蛋白质组分析揭示条斑紫菜突变体的生长机制
DOI：
10.1016/j.algal.2021.102420
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发表时间：
2021-10
影响因子：
5.1
通讯作者：
Wang Guangce
中科院分区：
文献类型：
--
作者：
Ma Yingchao;He Bangxiang;Wang Xulei;He Linwen;Niu Jianfeng;Huan Li;Lu Xiaoping;Xie Xiujun;Wang Guangce
前期研究表明，条斑紫菜突变株E菌体与初始菌株NA相比具有生长优势，转录组分析揭示了改善生长的相关生物过程。为了研究蛋白质水平的生长机制，本研究使用同量异位标签进行相对和绝对定量，并使用液相色谱-质谱法分析两种菌株的蛋白质组。共鉴定出277个蛋白，其中筛选出41个差异表达蛋白，其中上调蛋白22个，下调蛋白19个。蛋白质组和前期转录组的整合分析表明，突变株E中与光合作用、蛋白质生物合成、糖酵解和磷酸戊糖途径相关的蛋白质增强，这些蛋白质可能有利于突变株E的生长；与抗逆相关的蛋白质上调，可能促进突变株E在非生物胁迫下的生长，而与甲基化相关的蛋白质减少，可能增强突变株E中生长相关基因的表达。本研究通过整合揭示了突变株E的生长机制。组学分析，也为Py的分子育种提供理论基础。条斑鱼。
The previous study revealed that the mutant strain E thalli ofPyropia yezoensishad growth advantage compared with the initial strain NA, and transcriptome analysis revealed the relevant bioprocesses for improved growth. To investigate the growth mechanism at the protein level, this study used isobaric tags for relative and absolute quantification and liquid chromatography-mass spectrometry to analyze proteome of both strains. In total, 277 proteins were identified, among which 41 differentially expressed proteins were screened, including 22 up-regulated proteins and 19 down-regulated proteins. Integrated analysis of proteome and previous transcriptome showed that the proteins related to photosynthesis, protein biosynthesis, glycolysis, and pentose phosphate pathway were enhanced in mutant strain E, which were supposed to facilitate growth of mutant strain E, and the proteins relevant to stress resistance were up-regulated, which might promote the growth of mutant strain E under abiotic stresses, while proteins related to methylation were decreased, which might enhance the expression of growth relevant genes in mutant strain E. This study revealed the growth mechanism in mutant strain E by integrated omics analysis, and also provided theoretical foundation for molecular breeding ofPy. yezoensis.
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影响因子：
5.1
作者：
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通讯作者：
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