Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration.
Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration.
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Atoh1 诱导的异位再生后毛细胞发育中 ADF/destrin 表达的变化。
DOI：
10.3892/etm.2013.1089
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发表时间：
2013-07
影响因子：
2.7
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Jin K;Ren DD;Chi FL;Yang JM;Huang YB;Li W
本研究的目的是探讨肌动蛋白解聚因子（ADF）/destrin和激动体的位置变化在Atoh 1诱导的小鼠基底膜异位再生后毛细胞发育中的作用。通过免疫荧光法观察腺病毒转染、Atoh 1过表达和体外培养诱导毛细胞异位再生后不同时间点ADF/destrin和特异性激动体标志物γ-tubulin的表达。Atoh 1诱导异位再生后体外培养的新生毛细胞[Myo 7a（+）]中ADF/destrin分布和动力体位置的变化与正常发育过程中耳蜗感觉上皮毛细胞中ADF/destrin表达和动力体极性迁移的变化一致。ADF/destrin参与哺乳动物听觉上皮的发育以及异位再生毛细胞的发育和结构重排。Atoh 1诱导的异位再生后毛细胞的动力体在体外不同时间点显示出位置变化。
The aim of this study was to investigate the effects of actin depolymerizing factor (ADF)/destrin and position changes of kinetosomes in the development of hair cells following Atoh1-induced ectopic regeneration in the basilar membrane of mice. We observed through immunofluorescence at various time-points the expression of ADF/destrin and the specific kinetosome marker, γ-tubulin, in hair cells following ectopic regeneration induced by adenovirus transfection, overexpression of Atoh1 and in vitro culture. Changes of ADF/destrin distribution and kinetosome position during in vitro culture of new hair cells [Myo7a(+)] following Atoh1-induced ectopic regeneration are consistent with the changes in ADF/destrin expression and the polar migration of kinetosomes in hair cells of the cochlear sensory epithelium in normal development. ADF/destrin is involved in the development of the auditory epithelium and the development and structural rearrangement of ectopically regenerated hair cells in mammals. The kinetosomes of hair cells following Atoh1-induced ectopic regeneration show positional changes in vitro at different time-points.
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影响因子：
2.7
作者：
Pham, Hung;Yu, Hui;Laski, Frank A.
通讯作者：
Laski, Frank A.
影响因子：
9.2
作者：
Manor, Uri;Disanza, Andrea;Grati, M'Hamed;Andrade, Leonardo;Lin, Harrison;Di Fiore, Pier Paolo;Scita, Giorgio;Kachar, Bechara
通讯作者：
Kachar, Bechara
影响因子：
3.4
作者：
Matsumoto, Nozomu;Kitani, Rei;Kalinec, Federico
通讯作者：
Kalinec, Federico
影响因子：
19
作者：
Bernstein, Barbara W.;Bamburg, James R.
通讯作者：
Bamburg, James R.
DOI：
10.1083/jcb.201001138
发表时间：
2010-07-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Schwander M;Kachar B;Müller U
通讯作者：
Müller U