Identification of Candidate Iron Transporters From the ZIP/ZnT Gene Families in the Mosquito Aedes aegypti.
Identification of Candidate Iron Transporters From the ZIP/ZnT Gene Families in the Mosquito Aedes aegypti.
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DOI：
10.3389/fphys.2018.00380
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发表时间：
2018
影响因子：
4
通讯作者：
Adelman ZN
中科院分区：
文献类型：
--
作者：
Tsujimoto H;Anderson MAE;Myles KM;Adelman ZN
蚊子传播的病毒病原体，例如登革热和寨卡病毒，每年困扰着数万人。这些病毒在蚊子繁殖所需的吸血过程中传播，最重要的媒介是埃及伊蚊。虽然脊椎动物的血液富含蛋白质，但其高铁含量对蚊子可能有毒。尽管铁转运和封存对于媒介蚊子的繁殖至关重要，但我们发现蚊子缺乏常见铁转运蛋白 NRAMP 的同源物。使用一种新型的基于细胞的筛选，我们确定了埃及伊蚊（登革热、寨卡病毒和基孔肯雅热的传播媒介）中的两个 ZIP 和一个 ZnT 基因作为候选铁转运蛋白。我们确定了这些基因在早期繁殖关键时间点的器官特异性表达模式。结果表明这些基因在血液喂养时受到调节，特别是 ZIP13 同源物在血液喂养后高度上调，表明其在血液消化和繁殖过程中铁动员的重要性。基因沉默导致中肠和卵巢中铁积累的差异。该研究为进一步研究该病毒载体的铁转运和控制策略奠定了基础。
Mosquito-transmitted viral pathogens, such as dengue and Zika, afflict tens of thousands of people every year. These viruses are transmitted during the blood-feeding process that is required for mosquito reproduction, the most important vector being Aedes aegypti. While vertebrate blood is rich in protein, its high iron content is potentially toxic to mosquitoes. Although iron transport and sequestration are essential in the reproduction of vector mosquitoes, we discovered that culicine mosquitoes lack homologs of the common iron transporter NRAMP. Using a novel cell-based screen, we identified two ZIP and one ZnT genes as candidate iron transporters in the mosquito A. aegypti, the vector of dengue, Zika, and chikungunya. We determined the organ-specific expression pattern of these genes at critical time points in early reproduction. The result indicates modulation of these genes upon blood feeding, especially a ZIP13 homolog that is highly up-regulated after blood feeding, suggesting its importance in iron mobilization during blood digestion and reproduction. Gene silencing resulted in differential iron accumulation in the midgut and ovaries. This study sets a foundation for further investigation of iron transport and control strategies of this viral vector.
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DOI：
10.1016/j.bbagen.2010.03.004
发表时间：
2010-08
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Pham DQ;Winzerling JJ
通讯作者：
Winzerling JJ
影响因子：
2.8
作者：
Powell JR;Tabachnick WJ
通讯作者：
Tabachnick WJ
影响因子：
11.8
作者：
Macia, Eric;Ehrlich, Marcelo;Kirchhausen, Tomas
通讯作者：
Kirchhausen, Tomas
影响因子：
3.8
作者：
Martinez-Barnetche, Jesus;Garcia Solache, Monica;Lanz-Mendoza, Humberto
通讯作者：
Lanz-Mendoza, Humberto
影响因子：
3.7
作者：
Navarro JA;Schneuwly S
通讯作者：
Schneuwly S