Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion.
Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion.
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DOI：
10.1016/j.aca.2014.08.001
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发表时间：
2014-10-27
影响因子：
6.2
通讯作者：
Cooks RG
中科院分区：
文献类型：
--
作者：
Pirro V;Oliveri P;Ferreira CR;González-Serrano AF;Machaty Z;Cooks RG
分析单个细胞的灵敏测量的发展与阐明每个细胞在生理和病理条件下的特殊作用或代谢功能相关。脂质在细胞功能中发挥着多种关键作用，分析方法在脂质组学领域的应用越来越受到人们的关注。在这项工作中，研究了猪卵母细胞的体外成熟。通过使用解吸电喷雾电离质谱 (DESI-MS) 获得来自单个卵母细胞（未成熟卵母细胞、24 小时和 44 小时体外成熟卵母细胞）的两个独立的化学信息来源（以正离子模式和负离子模式表示的质谱）。提出了低水平和中水平数据融合策略，旨在更好地探索两种质谱脂质谱中包含的大量化学信息。通过主成分分析（PCA）在两种多块方法中探索数据，包括游离脂肪酸、磷脂、胆固醇相关分子、二酰基甘油和三酰基甘油的信息。数据融合后，观察到未成熟和体外成熟猪卵母细胞之间更明显的差异，这提供了有关卵母细胞成熟过程中脂质代谢的新信息。特别是，在体外成熟过程中，TAG 组成的变化以及脂肪酸代谢和膜复杂性的增加得到了证实。这些信息可以帮助改善猪种的体外胚胎生产。
The development of sensitive measurements to analyze individual cells is of relevance to elucidate specialized roles or metabolic functions of each cell under physiological and pathological conditions. Lipids play multiple and critical roles in cellular functions and the application of analytical methods in the lipidomics area is of increasing interest. In this work, in vitro maturation of porcine oocytes was studied. Two independent sources of chemical information (represented by mass spectra in the positive and negative ion modes) from single oocytes (immature oocytes, 24-hour and 44-hour in vitro matured oocytes) were acquired by using desorption electrospray ionization-mass spectrometry (DESI-MS). Low and mid-level data fusion strategies are presented with the aim of better exploring the large amount of chemical information contained in the two mass spectrometric lipid profiles. Data were explored by principal component analysis (PCA) within the two multi-block approaches to include information on free fatty acids, phospholipids, cholesterol-related molecules, di- and triacylglycerols. After data fusion, clearer differences among immature and in vitro matured porcine oocytes were observed, which provide novel information regarding lipid metabolism throughout oocyte maturation. In particular, changes in TAG composition, as well as increase in fatty acid metabolism and membrane complexity were evidenced during the in vitro maturation process. This information can assist the improvement of in vitro embryo production for porcine species.
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影响因子：
4.3
作者：
Ferreira, Christina R.;Pirro, Valentina;Cooks, R. Graham
通讯作者：
Cooks, R. Graham
影响因子：
3.7
作者：
González-Serrano AF;Pirro V;Ferreira CR;Oliveri P;Eberlin LS;Heinzmann J;Lucas-Hahn A;Niemann H;Cooks RG
通讯作者：
Cooks RG
影响因子：
6.5
作者：
Ferreira, Christina R.;Saraiva, Sergio A.;Eberlin, Marcos N.
通讯作者：
Eberlin, Marcos N.
DOI：
10.1016/j.chemolab.2006.05.002
发表时间：
2007-01-15
影响因子：
3.9
作者：
Forshed, Jenny;Idborg, Helena;Jacobsson, Sven P.
通讯作者：
Jacobsson, Sven P.
DOI：
10.1002/mrd.1120240205
发表时间：
1989-10-01
期刊：
GAMETE RESEARCH
影响因子：
--
作者：
COLONNA, R;TATONE, C;MANGIA, F
通讯作者：
MANGIA, F