The actin cytoskeletal network plays a role in yeast prion transmission and contributes to prion stability.
The actin cytoskeletal network plays a role in yeast prion transmission and contributes to prion stability.
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DOI：
10.1111/mmi.14528
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发表时间：
2020-09
影响因子：
3.6
通讯作者：
Manogaran AL
中科院分区：
文献类型：
--
作者：
Dorweiler JE;Oddo MJ;Lyke DR;Reilly JA;Wisniewski BT;Davis EE;Kuborn AM;Merrill SJ;Manogaran AL
从已有的朊病毒聚集体中剪切和产生可传播的传播体需要伴侣网络。然而，维持酵母菌朊病毒所需的其他细胞网络在很大程度上是未知的。在这里，我们建立了肌动蛋白网络在朊病毒维持中的新作用。[PIN+]朊病毒，也被称为[RNQ+]，在细胞分裂和细胞质转移过程中，作为稳定的变体依赖于伴侣蛋白机制将繁殖体传递给子细胞。Hsp104分子伴侣的缺失导致朊病毒颗粒的生长，直到它们太大而无法传播。在这里，我们分离了一个独特的[PIN+]变体，它在肌动蛋白突变体中是不稳定的。这种朊病毒的丢失可以在许多代中观察到，并且与Rnq1的高分子量物种和在细胞分裂过程中不对称保留的大可见聚集体的检测相一致。我们的数据表明，在这些突变体中发现的不规则肌动蛋白网络可能会随着时间的推移缓慢地允许聚集体生长，从而影响繁殖数量，导致产生不可传播的大聚集体。因此，我们展示了细胞骨架网络在朊病毒传播中的潜在贡献，这与神经退行性蛋白聚集疾病中小淀粉样蛋白细胞间传播的模型相似。本研究确立了肌动蛋白网络在朊病毒维持中的新作用。一种独特的[PIN+]朊病毒变体在肌动蛋白突变体中是不稳定的，该突变体在一个保守的肌动蛋白结合蛋白Fimbrin的结合位点上携带突变。我们的数据表明，随着时间的推移，不规则的肌动蛋白网络可以通过缓慢地允许总增长来影响传播数，从而导致非传播传播的产生。
Chaperone networks are required for the shearing and generation of transmissible propagons from pre-existing prion aggregates. However, other cellular networks needed for maintaining yeast prions are largely uncharacterized. Here, we establish a novel role for actin networks in prion maintenance. The [PIN+] prion, also known as [RNQ+], exists as stable variants dependent upon the chaperone machinery for the transmission of propagons to daughter cells during cell division and cytoplasmic transfer. Loss of the Hsp104 molecular chaperone leads to the growth of prion particles until they are too large to be transmitted. Here, we isolated a unique [PIN+] variant, which is unstable in actin mutants. This prion loss is observed over many generations, and coincides with the detection of both high molecular weight species of Rnq1 and large visible aggregates that are asymmetrically retained during cell division. Our data suggest that the irregular actin networks found in these mutants may influence propagon number by slowly permitting aggregate growth over time, resulting in the generation of non-transmissible large aggregates. Thus, we show the potential contribution of cytoskeletal networks in the transmission of prion propagons, which parallels models that have been proposed for cell-to-cell transmission of small amyloids in neurodegenerative protein aggregation diseases. This study establishes a novel role for actin networks in prion maintenance. A unique variant of the [PIN+] prion is unstable in actin mutants carrying mutations within a binding site for Fimbrin, a conserved actin bundling protein. Our data suggest that the irregular actin networks can influence propagon number by slowly permitting aggregate growth over time, resulting in the generation of non-transmissible propagons.
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影响因子：
7.8
作者：
HONTS, JE;SANDROCK, TS;ADAMS, AEM
通讯作者：
ADAMS, AEM
DOI：
10.1038/nsb0195-28
发表时间：
1995-01-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
AMBERG, DC;BASART, E;BOTSTEIN, D
通讯作者：
BOTSTEIN, D
DOI：
10.3390/pathogens7010005
发表时间：
2018-01-01
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Igel-Egalon A;Béringue V;Rezaei H;Sibille P
通讯作者：
Sibille P
新发现的朊病毒与酿酒酵母中染色质重塑因子 Swi1 相关
影响因子：
30.8
作者：
Du, Zhiqiang;Park, Kyung-Won;Li, Liming
通讯作者：
Li, Liming
DOI：
10.1073/pnas.0308413101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Fevrier, B;Vilette, D;Raposo, G
通讯作者：
Raposo, G