Daily HIV pre-exposure prophylaxis (PrEP) with tenofovir disoproxil fumarate-emtricitabine reduced Streptococcus and increased Erysipelotrichaceae in rectal microbiota.
Daily HIV pre-exposure prophylaxis (PrEP) with tenofovir disoproxil fumarate-emtricitabine reduced Streptococcus and increased Erysipelotrichaceae in rectal microbiota.
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DOI：
10.1038/s41598-018-33524-6
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发表时间：
2018-10-12
影响因子：
4.6
通讯作者：
Lee HY
中科院分区：
文献类型：
--
作者：
Dubé MP;Park SY;Ross H;Love TMT;Morris SR;Lee HY
每日 PrEP 对于预防 HIV-1 感染非常有效，但长期服用富马酸替诺福韦二吡呋酯加恩曲他滨 (TDF-FTC) 的风险除了最初的胃肠道副作用外，还包括肾功能衰退和骨矿物质密度降低。我们使用 PrEP 开始前和坚持使用 PrEP 48 至 72 周后从健康 MSM 收集的直肠拭子研究了 TDF-FTC 对肠道微生物组的影响。 16S 核糖体 RNA 基因测序的 V4 区显示，每日 PrEP 48-72 周后，链球菌从 12.0% 显着减少至 1.2% (p = 0.036)，丹毒科从 0.79% 显着增加至 3.3% (p = 0.028)。丹毒科内的三尾链状杆菌、双形霍德曼氏菌和血图里奇杆菌增加，无乳链球菌、口腔链球菌、轻链球菌减少。这些变化与宿主因素无关，包括 PrEP 持续时间、年龄、种族、二磷酸替诺福韦血液浓度、任何药物使用和药物滥用，表明观察到的微生物组变化可能是由每日 PrEP 使用引起的。长期 PrEP 会导致三尾链状杆菌和二形霍德曼氏菌增加，这些细菌与肠道微生物群失调有关。我们的观察结果可以帮助描述 PrEP 的副作用，这可能会提高 PrEP 的依从性，从而预防 HIV-1。
Daily PrEP is highly effective at preventing HIV-1 acquisition, but risks of long-term tenofovir disoproxil fumarate plus emtricitabine (TDF-FTC) include renal decline and bone mineral density decrease in addition to initial gastrointestinal side effects. We investigated the impact of TDF-FTC on the enteric microbiome using rectal swabs collected from healthy MSM before PrEP initiation and after 48 to 72 weeks of adherent PrEP use. The V4 region of the 16S ribosomal RNA gene sequencing showed that Streptococcus was significantly reduced from 12.0% to 1.2% (p = 0.036) and Erysipelotrichaceae family was significantly increased from 0.79% to 3.3% (p = 0.028) after 48–72 weeks of daily PrEP. Catenibacterium mitsuokai, Holdemanella biformis and Turicibacter sanguinis were increased within the Erysipelotrichaceae family and Streptococcus agalactiae, Streptococcus oralis, Streptococcus mitis were reduced. These changes were not associated with host factors including PrEP duration, age, race, tenofovir diphosphate blood level, any drug use and drug abuse, suggesting that the observed microbiome shifts were likely induced by daily PrEP use. Long-term PrEP resulted in increases of Catenibacterium mitsuokai and Holdemanella biformis, which have been associated with gut microbiome dysbiosis. Our observations can aid in characterizing PrEP’s side effects, which is likely to improve PrEP adherence, and thus HIV-1 prevention.
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影响因子：
6.4
作者：
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通讯作者：
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通讯作者：
Grant, Robert M.