Molecular Characterization of a Geranyl Diphosphate-Specific Prenyltransferase Catalyzing Stilbenoid Prenylation from Morus alba
Molecular Characterization of a Geranyl Diphosphate-Specific Prenyltransferase Catalyzing Stilbenoid Prenylation from Morus alba
复制标题
香叶基二磷酸特异性异戊二烯基转移酶催化桑白芪类异戊二烯化的分子表征
DOI：
10.1093/pcp/pcy138
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发表时间：
2018-07
影响因子：
4.9
通讯作者：
Jingkui Tian
中科院分区：
文献类型：
--
作者：
Zhuoheng Zhong;Wei Zhu;Shengzhi Liu;Qijie Guan;Xi Chen;Wei Huang;Tantan Wang;Bingxian Yang;Jingkui Tian
药用植物中具有药用活性的化合物是新药开发的重要来源。亲脂性增强的戊烯基二苯乙烯是具有广泛生物活性的重要次生代谢产物。到目前为止，已经从桑树中分离出许多戊烯基二苯乙烯类化合物，但负责关键的戊烯基修饰的酶尚不清楚。在本研究中，鉴定了一种名为桑白藜芦醇香叶转移酶（MaOGT）的二苯乙烯类特异性戊烯基转移酶（PT），并对其进行了体外功能表征。MaOGT将氧化白藜芦醇和二磷酸香叶醇（GPP）识别为天然底物，并催化氧化白藜芦醇戊酰化。结果表明，MaOGT具有多个跨膜区、保守功能域和靶向植物质体等特征。这个独特的PT代表了第一个接受GPP作为天然戊烯基供体的二苯乙烯类特异性PT，并且可以帮助鉴定其他功能不同的灰质植物PT。此外，MaOGT可用于高效、大规模的氧化白藜芦醇烯酰化制备具有潜在治疗价值的生物活性化合物。
Pharmaceutically active compounds from medical plants are attractive as a major source for new drug development. Prenylated stilbenoids with increased lipophilicity are valuable secondary metabolites which possess a wide range of biological activities. So far, many prenylated stilbenoids have been isolated from Morus alba but the enzyme responsible for the crucial prenyl modification remains unknown. In the present study, a stilbenoid-specific prenyltransferase (PT), termed Morus alba oxyresveratrol geranyltransferase (MaOGT), was identified and functionally characterized in vitro. MaOGT recognized oxyresveratrol and geranyl diphosphate (GPP) as natural substrates, and catalyzed oxyresveratrol prenylation. Our results indicated that MaOGT shared common features with other aromatic PTs, e.g. multiple transmembrane regions, conserved functional domains and targeting to plant plastids. This distinct PT represents the first stilbenoid-specific PT accepting GPP as a natural prenyl donor, and could help identify additional functionally varied PTs in moraceous plants. Furthermore, MaOGT might be applied for high-efficiency and large-scale prenylation of oxyresveratrol to produce bioactive compounds for potential therapeutic applications.
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影响因子：
7.4
作者：
Tianhong Yang;Lingling Fang;A. Rimando;V. Sobolev;K. Mockaitis;F. Medina-Bolivar
通讯作者：
Tianhong Yang;Lingling Fang;A. Rimando;V. Sobolev;K. Mockaitis;F. Medina-Bolivar
影响因子：
2.8
作者：
E. Reimann
通讯作者：
E. Reimann
影响因子：
5.2
作者：
Raju, Ritesh;Piggott, Andrew M.;Capon, Robert J.
通讯作者：
Capon, Robert J.
DOI：
10.1074/jbc.ra117.000564
发表时间：
2017-11
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
Tianhong Yang;Lingling Fang;Sheri Sanders;S. Jayanthi;Gayathri Rajan;Ram Podicheti;S. Thallapuranam;K. Mockaitis;F. Medina-Bolivar
通讯作者：
Tianhong Yang;Lingling Fang;Sheri Sanders;S. Jayanthi;Gayathri Rajan;Ram Podicheti;S. Thallapuranam;K. Mockaitis;F. Medina-Bolivar
影响因子：
7.4
作者：
Sasaki, Kanako;Mito, Kouji;Yazaki, Kazufumi
通讯作者：
Yazaki, Kazufumi