Anchorage-independent colony formation of SV40 transformed BALB/c-3T3 cells in serum-free medium: Role of cell- and serum-derived factors
Anchorage-independent colony formation of SV40 transformed BALB/c-3T3 cells in serum-free medium: Role of cell- and serum-derived factors
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无血清培养基中 SV40 转化的 BALB/c-3T3 细胞的贴壁独立集落形成：细胞和血清衍生因子的作用
DOI：
10.1016/0092-8674(83)90084-3
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发表时间：
1983
期刊：
影响因子：
64.5
通讯作者：
D. Mcclure
中科院分区：
文献类型：
--
作者：
D. Mcclure
在无血清培养基（胰岛素、转铁蛋白和血清白蛋白作为唯一的大分子补充剂）中研究了琼脂糖悬浮培养物中 SV40 转化的 BALB/c-3T3 细胞的集落形成反应，该培养基针对纤连蛋白附着的单层培养物的集落形成进行了优化。在这种无血清培养基中，SV3T3 细胞无法在琼脂糖悬浮液中形成集落。然而，它们可以通过生长因子诱导形成不依赖贴壁的集落形成，而生长因子是其未转化对应物在单层培养物中增殖所额外需要的。 SV3T3 细胞还通过来自致密单层无血清 SV3T3 培养物的条件培养基，在无血清培养基中形成不贴壁独立的集落。这些实验表明，细胞与基质的相互作用决定了纤连蛋白附着的转化细胞的生长因子自主性。
The colony-forming response of SV40 transformed BALB/c-3T3 cells in agarose suspension culture was studied in a serum-free medium (with insulin, transferrin and serum albumin as the only macromolecular supplements) that was optimized for colony formation of fibronectin-attached monolayer cultures. In this serum-free medium, the SV3T3 cells fail to form colonies in agarose suspension. However, they can be induced to anchorage-independent colony formation by the growth factors that are additionally required by their untransformed counterparts for proliferation in monolayer culture. The SV3T3 cells are also rendered anchorage-independent for colony formation in serum-free medium by conditioned medium from dense monolayer serumfree SV3T3 cultures. These experiments suggest that it is the cell-substrate interaction that is responsible for the growth factor autonomy of fibronectin-attached transformed cells.
影响因子：
3.7
作者：
Kaplan,PL;Topp,WC;Ozanne,B
通讯作者：
Ozanne,B
DOI：
10.1002/jss.400140106
发表时间：
1980-01-01
期刊：
JOURNAL OF SUPRAMOLECULAR STRUCTURE
影响因子：
--
作者：
BARNES, D;WOLFE, R;SATO, G
通讯作者：
SATO, G