The Role of Genetically Modified Human Feeder Cells in Maintaining the Integrity of Primary Cultured Human Deciduous Dental Pulp Cells.
The Role of Genetically Modified Human Feeder Cells in Maintaining the Integrity of Primary Cultured Human Deciduous Dental Pulp Cells.
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DOI：
10.3390/jcm11206087
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发表时间：
2022-10-15
影响因子：
3.9
通讯作者：
Saitoh I
中科院分区：
文献类型：
--
作者：
Ibano N;Inada E;Otake S;Kiyokawa Y;Sakata K;Sato M;Kubota N;Noguchi H;Iwase Y;Murakami T;Sawami T;Kakihara Y;Maeda T;Terunuma M;Terao Y;Saitoh I
组织特异性干细胞存在于组织和器官中，例如皮肤和骨髓。然而，与胚胎干细胞相比，它们的多能性有限。在塑料组织培养皿上培养原代细胞可能会导致多能性丧失，因为组织特异性干细胞无法在无饲养层培养皿中存活。最近的研究结果表明，在含有饲养细胞的培养基中培养原代细胞，特别是表达生长因子的转基因饲养细胞，可能有利于它们的生存和增殖。因此，本研究的目的是阐明表达生长因子的转基因人类饲养细胞在维持原代培养的人类乳牙牙髓细胞完整性中的作用。 PCR 证明，表达白血病抑制因子、骨形态发生蛋白 4 和碱性成纤维细胞生长因子的饲养细胞已成功改造。与丝裂霉素C处理的饲养细胞共培养增强了新分离的人乳牙牙髓细胞的增殖，促进其分化为脂肪细胞和神经元，并保持其干性特性。我们的研究结果表明，转基因人类饲养细胞可用于维持原代培养的人类乳牙牙髓细胞的完整性。
Tissue-specific stem cells exist in tissues and organs, such as skin and bone marrow. However, their pluripotency is limited compared to embryonic stem cells. Culturing primary cells on plastic tissue culture dishes can result in the loss of multipotency, because of the inability of tissue-specific stem cells to survive in feeder-less dishes. Recent findings suggest that culturing primary cells in medium containing feeder cells, particularly genetically modified feeder cells expressing growth factors, may be beneficial for their survival and proliferation. Therefore, the aim of this study was to elucidate the role of genetically modified human feeder cells expressing growth factors in maintaining the integrity of primary cultured human deciduous dental pulp cells. Feeder cells expressing leukemia inhibitory factor, bone morphogenetic protein 4, and basic fibroblast growth factor were successfully engineered, as evidenced by PCR. Co-culturing with mitomycin-C-treated feeder cells enhanced the proliferation of newly isolated human deciduous dental pulp cells, promoted their differentiation into adipocytes and neurons, and maintained their stemness properties. Our findings suggest that genetically modified human feeder cells may be used to maintain the integrity of primary cultured human deciduous dental pulp cells.
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