MicroRNA expression differentiates squamous epithelium from Barrett's esophagus and esophageal cancer.
MicroRNA expression differentiates squamous epithelium from Barrett's esophagus and esophageal cancer.
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DOI：
10.1007/s10620-013-2806-7
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发表时间：
2013-11
影响因子：
3.1
通讯作者：
Shaheen, Nicholas J.
中科院分区：
文献类型：
--
作者：
Garman, Katherine S.;Owzar, Kouros;Hauser, Elizabeth R.;Westfall, Kristen;Anderson, Blair R.;Souza, Rhonda F.;Diehl, Anna Mae;Provenzale, Dawn;Shaheen, Nicholas J.
目前的策略未能在疾病进展到无法治愈之前识别大多数食管腺癌(EAC)患者。鉴于与EAC相关的黯淡预后，需要改进早期食道肿瘤的检测。我们试图评估microRNAs的差异表达是否可以区分鳞状上皮、Barrett‘s食道(BE)和EAC。我们分析了36例患者的胃镜活检样本中microRNA的表达，这些患者代表正常鳞状食道(n=11)、BE(n=14)和高度不典型增生(HGD)/EAC(n=11)。使用代表847个人类microRNAs的微阵列评估RNA，然后对9个microRNAs进行qRT-PCR验证。在第二个队列(n=18)中，对5个miRNAs进行了qRT-PCR验证。在我们的培训队列中评估了文献中与BE/EAC相关的59个microRNAs的表达。已知的食道细胞系被用来比较miRNA的表达与组织的miRNAs。在控制多重比较后，通过微阵列分析，我们发现34个miRNAs在鳞状食道和BE/EAC中差异表达。然而，miRNA的表达并不能可靠地区分非发育不良的BE和EAC。在验证队列中，选择进行qRT-PCR验证的所有五个microRNAs在鳞状样本和BE/EAC之间存在差异。微阵列结果支持文献中与BE/EAC相关的14个先前报道的microRNAs。细胞系一般不反映体内发现的miRNA表达。这些数据表明，食管鳞状上皮和BE/EAC之间的miRNAs不同，但不能区分BE和EAC。我们建议对EAC高危患者的miRNAs进行前瞻性评估。
Current strategies fail to identify most patients with esophageal adenocarcinoma (EAC) before the disease becomes advanced and incurable. Given the dismal prognosis associated with EAC, improvements in detection of early-stage esophageal neoplasia are needed. We sought to assess whether differential expression of microRNAs could discriminate between squamous epithelium, Barrett’s esophagus (BE), and EAC. We analyzed microRNA expression in a discovery cohort of human endoscopic biopsy samples from 36 patients representing normal squamous esophagus (n=11), BE (n=14), and high-grade dysplasia (HGD)/EAC (n=11). RNA was assessed using microarrays representing 847 human microRNAs followed by qRT-PCR verification of nine microRNAs. In a second cohort (n=18), qRT-PCR validation of five miRNAs was performed. Expression of 59 microRNAs associated with BE/EAC in the literature was assessed in our training cohort. Known esophageal cell lines were used to compare miRNA expression to tissue miRNAs. After controlling for multiple comparisons, we found 34 miRNAs differentially expressed between squamous esophagus and BE/EAC by microarray analysis. However, miRNA expression did not reliably differentiate non-dysplastic BE from EAC. In the validation cohort, all five microRNAs selected for qRT-PCR validation differentiated between squamous samples and BE/EAC. Microarray results supported 14 of the previously reported microRNAs associated with BE/EAC in the literature. Cell lines did not generally reflect miRNA expression found in vivo. These data indicate that miRNAs differ between squamous esophageal epithelium and BE/EAC, but do not distinguish between BE and EAC. We suggest prospective evaluation of miRNAs in patients at high risk for EAC.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
9.8
作者：
Bansal, Ajay;Lee, I. H.;Christenson, L. K.
通讯作者：
Christenson, L. K.
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ