Biological Characteristics and Osteogenic Differentiation of Ovine Bone Marrow Derived Mesenchymal Stem Cells Stimulated with FGF-2 and BMP-2.
Biological Characteristics and Osteogenic Differentiation of Ovine Bone Marrow Derived Mesenchymal Stem Cells Stimulated with FGF-2 and BMP-2.
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DOI：
10.3390/ijms21249726
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发表时间：
2020-12-20
影响因子：
5.6
通讯作者：
Klimczak A
中科院分区：
文献类型：
--
作者：
Gromolak S;Krawczenko A;Antończyk A;Buczak K;Kiełbowicz Z;Klimczak A
利用间充质干细胞（MSCs）的细胞为基础的治疗是骨组织工程的一个有前途的工具。骨髓间充质干细胞的骨再生涉及一系列的分子过程，导致由生物活性因子支持的骨诱导级联反应的激活，生物活性因子包括成纤维细胞生长因子-2（FGF-2）和骨形态发生蛋白-2（BMP-2）。在这项研究中，我们研究了羊骨髓间充质干细胞（BM-MSCs）的生物学特性和成骨分化的潜力与20 ng/mL的FGF-2和100 ng/mL的BMP-2在体外处理。成骨诱导的BM-MSCs的生物学特性进行了研究，通过评估其形态，增殖，表型和细胞因子分泌谱。通过茜素红S染色、骨钙素和I型胶原的免疫荧光染色以及成骨遗传标记物的表达水平来表征成骨分化。结果表明，用FGF-2和BMP-2处理的BM-MSC保持了它们的原代MSC特性，并提高了它们的成骨分化能力，如通过骨钙素和I型胶原蛋白的表达增加以及成骨相关基因标记物BMP-2、Runx 2、osterix、I型胶原蛋白、骨钙素和骨桥蛋白的上调所证实的。此外，绵羊BM-MSCs产生了多种参与成骨的生物活性因子，并且补充FGF-2和BMP-2的培养基影响了细胞的分泌组特征。结果表明，绵羊成骨诱导的BM-MSCs可以作为一种细胞疗法，在临床前大动物模型中研究骨修复。
Cell-based therapies using mesenchymal stem cells (MSCs) are a promising tool in bone tissue engineering. Bone regeneration with MSCs involves a series of molecular processes leading to the activation of the osteoinductive cascade supported by bioactive factors, including fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2). In this study, we examined the biological characteristics and osteogenic differentiation potential of sheep bone marrow MSCs (BM-MSCs) treated with 20 ng/mL of FGF-2 and 100 ng/mL BMP-2 in vitro. The biological properties of osteogenic-induced BM-MSCs were investigated by assessing their morphology, proliferation, phenotype, and cytokine secretory profile. The osteogenic differentiation was characterized by Alizarin Red S staining, immunofluorescent staining of osteocalcin and collagen type I, and expression levels of genetic markers of osteogenesis. The results demonstrated that BM-MSCs treated with FGF-2 and BMP-2 maintained their primary MSC properties and improved their osteogenic differentiation capacity, as confirmed by increased expression of osteocalcin and collagen type I and upregulation of osteogenic-related gene markers BMP-2, Runx2, osterix, collagen type I, osteocalcin, and osteopontin. Furthermore, sheep BM-MSCs produced a variety of bioactive factors involved in osteogenesis, and supplementation of the culture medium with FGF-2 and BMP-2 affected the secretome profile of the cells. The results suggest that sheep osteogenic-induced BM-MSCs may be used as a cellular therapy to study bone repair in the preclinical large animal model.
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影响因子：
8.1
作者：
Charoenlarp P;Rajendran AK;Iseki S
通讯作者：
Iseki S
影响因子：
5.6
作者：
Cartland SP;Genner SW;Zahoor A;Kavurma MM
通讯作者：
Kavurma MM
影响因子：
4.8
作者：
Kim, HJ;Kim, JH;Ryoo, HM
通讯作者：
Ryoo, HM
影响因子：
5.6
作者：
Haddouti, El-Mustapha;Randau, Thomas M.;Schildberg, Frank A.
通讯作者：
Schildberg, Frank A.
影响因子：
2.8
作者：
Kawaguchi, Hiroshi;Jingushi, Seiya;Nakamura, Kozo
通讯作者：
Nakamura, Kozo