Alternative 3' UTRs play a widespread role in translation-independent mRNA association with the endoplasmic reticulum.
Alternative 3' UTRs play a widespread role in translation-independent mRNA association with the endoplasmic reticulum.
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DOI：
10.1016/j.celrep.2021.109407
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发表时间：
2021-07-20
期刊：
影响因子：
8.8
通讯作者：
Tian B
中科院分区：
文献类型：
--
作者：
Cheng LC;Zheng D;Zhang Q;Guvenek A;Cheng H;Tian B
编码膜和分泌蛋白的转录本通过翻译与内质网(ER)相联系。在这里，使用细胞分离、多聚体图谱和3‘端测序，我们表明转录本在翻译非依赖内质网关联(Tiera)方面有很大的不同。某些功能基团中的基因，如细胞信号转导，往往具有显著高于其他功能基团的Tiera潜能，这表明内质网关联对其mRNA代谢的重要性，如局部翻译。转录本的Tiera潜力主要由大小、序列含量和RNA结构决定。由于转录特征的变化，选择性多聚腺苷酸化(APA)亚型可以具有不同的Tiera潜力。细胞分化中广泛存在的3‘非编码区延长导致转录产物与内质网有更大的关联，尤其是那些能够表达很长3’非编码区的基因。我们的数据还表明，Tiera与翻译依赖的ER关联处于动态竞争中，这表明ER上用于mRNA关联的空间有限。程等人结果表明，不依赖翻译的内质网关联(Tiera)中的记录有很大的不同。某些功能基团中的转录本，如细胞信号，往往具有较高的Tiera潜力。选择性的聚腺苷酸化改变了决定Tiera的转录特征，导致了不同的内质网关联的mRNA亚型。
Transcripts encoding membrane and secreted proteins are known to associate with the endoplasmic reticulum (ER) through translation. Here, using cell fractionation, polysome profiling, and 3′ end sequencing, we show that transcripts differ substantially in translation-independent ER association (TiERA). Genes in certain functional groups, such as cell signaling, tend to have significantly higher TiERA potentials than others, suggesting the importance of ER association for their mRNA metabolism, such as localized translation. The TiERA potential of a transcript is determined largely by size, sequence content, and RNA structures. Alternative polyadenylation (APA) isoforms can have distinct TiERA potentials because of changes in transcript features. The widespread 3′ UTR lengthening in cell differentiation leads to greater transcript association with the ER, especially for genes that are capable of expressing very long 3′ UTRs. Our data also indicate that TiERA is in dynamic competition with translation-dependent ER association, suggesting limited space on the ER for mRNA association. Cheng et al. show that transcripts differ substantially in translation-independent ER association (TiERA). Transcripts in certain functional groups, such as cell signaling, tend to have high TiERA potentials. Alternative polyadenylation changes transcript features that determine TiERA, leading to distinct ER association of mRNA isoforms.
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影响因子：
64.5
作者：
Hogg JR;Goff SP
通讯作者：
Goff SP
影响因子：
16.2
作者：
Flavell, Steven W.;Kim, Tae-Kyung;Gray, Jesse M.;Harmin, David A.;Hemberg, Martin;Hong, Elizabeth J.;Markenscoff-Papadimitriou, Eirene;Bear, Daniel M.;Greenberg, Michael E.
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通讯作者：
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DOI：
10.3791/1948
发表时间：
2010-06-15
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
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通讯作者：
Kinzy, Terri G
影响因子：
9.8
作者：
Cui XA;Zhang H;Palazzo AF
通讯作者：
Palazzo AF