Two types of cholinergic innervation in cortex, one co-localized with vasoactive intestinal polypeptide-猫眼课题宝

皮质中两种类型的胆碱能神经支配，一种与血管活性肠多肽共定位

DOI：

发表时间：

1984

期刊：

Nature

影响因子：

64.8

通讯作者：

中科院分区：

综合性期刊1区

文献类型：

作者：

F. Eckenstein;R. Baughman

哺乳动物大脑皮层中胆碱能神经元细胞体的存在长期以来一直是一个备受争议的话题（参见参考文献 1 的评论）。然而，最近，通过免疫组织化学方法证明了一种特定的胆碱能标记物，乙酰胆碱合成酶，胆碱乙酰转移酶（ChAT，E.C.2.3.1.6）存在于大鼠皮质2,3的双极神经元中。在这里，我们表明，这些内在胆碱能神经元中至少 80% 还含有血管活性肠多肽 (VIP) 的免疫反应性，这是一种被发现存在于皮质神经元亚群中的神经活性肽4。另一方面，我们发现基底前脑中的 ChAT 阳性细胞（皮质 5-8 胆碱能神经支配的另一个主要来源）不包含可检测到的 VIP 免疫反应性。此外，我们通过光学和电子显微镜观察到，皮质中的一些VIP-和一些ChAT-阳性结构与血管密切相关。

The existence of cholinergic neuronal cell bodies in mammalian cerebral cortex was long the subject of much controversy (see ref. 1 for review). Recently, however, a specific cholinergic marker, the acetylcholine synthesizing enzyme, choline acetyltransferase (ChAT, E.C.2.3.1.6), was demonstrated by immunohistochemical methods to be present in bipolar neurones in rat cortex2,3. Here we show that at least 80% of these intrinsic cholinergic neurones also contain immunoreactivity for vasoactive intestinal polypeptide (VIP), a neuroactive peptde found to be present in a subpopulation of cortical neurones4. On the other hand, we find that the ChAT-positive cells in the basal forebrain, which are another major source of cholinergic innervation of the cortex5–8, contain no detectable VIP-immunoreactivity. In addition, we have observed by both light and electron microscopy that some VIP- and some ChAT-positive structures in cortex are closely associated with blood vessels.

Cholecystokinin-immunoreactive neurons in rat and monkey cerebral cortex make symmetric synapses and have intimate associations with blood vessels.