Electrospun Zein Fibrous Membranes Using Glyoxal as Cross-Linking Agent: Preparation, Characterization and Potential for Use in Biomedical Applications
Electrospun Zein Fibrous Membranes Using Glyoxal as Cross-Linking Agent: Preparation, Characterization and Potential for Use in Biomedical Applications
复制标题
使用乙二醛作为交联剂的电纺玉米醇溶蛋白纤维膜：制备、表征及其在生物医学应用中的应用潜力
DOI：
--
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Orawan Suwantong
中科院分区：
文献类型：
--
作者：
Orawan Suwantong
本研究采用静电纺丝法制备了玉米醇溶蛋白超细纤维。这些纤维的直径在0.32~1.10μm之间。通过在基础玉米醇溶蛋白溶液中添加不同数量的乙二醛水溶液(即，基于玉米醇溶蛋白溶液体积的0、5、10和15%v/v)(即，40%w/v)，制备了交联型电纺玉米醇溶蛋白纤维毡。乙二醛的存在使电纺玉米醇溶蛋白纤维毡的力学性能得到明显改善。用小鼠成纤维细胞(L929)间接细胞毒性评价和人包皮成纤维细胞(HFF)直接培养的方法评价了交联电纺玉米醇溶蛋白纤维垫作为细胞/组织培养支架材料的潜力。研究发现，乙二醛用量的增加会导致交联物的毒性增加，但这可以通过在甘氨酸水溶液中对交联型电纺玉米醇溶蛋白纤维垫进行前处理来缓解。最后，在部分交联型纤维毡上培养5d，细胞生长良好。
In the present contribution, ultra-fine zein fibers were successfully prepared by electrospinning. The diameters of these fibers ranged between 0.32 and 1.10 μm. Cross-linked electrospun zein fiber mats were prepared by adding varying quantities of glyoxal aqueous solution (i.e., 0, 5, 10 and 15% v/v based on the volume of the zein solution) into the base zein solution (i.e, 40% w/v). Mechanical properties of the electrospun zein fiber mats were evidently improved by the cross-linking with glyoxal. The fibrous nature of the crosslinked materials was preserved even after submersion in distilled water at room temperature (i.e., 25 1C) or 37C for 5 d. The potential for use of the cross-linked electrospun zein fiber mats as scaffolding materials for cell/tissue culture was assessed with indirect cytotoxicity evaluation with mouse fibroblasts (L929) and direct culture with human foreskin fibroblasts (HFF). It was found that an increase in the amount of glyoxal used to cross-link the materials caused the cross-linked materials to be more toxic, but this can be alleviated by pre-treatment of the cross-linked electrospun zein fiber mats in a glycine aqueous solution. Finally, the culture of HFF on some of the cross-linked fiber mat samples for 5 d showed that the cells grew well on the materials.
影响因子：
14
作者：
Li, MY;Guo, Y;Lelkes, PI
通讯作者：
Lelkes, PI