Acute salinity tolerance and the control of two prolactins and their receptors in the Nile tilapia (Oreochromis niloticus) and Mozambique tilapia (O. mossambicus): A comparative study.
Acute salinity tolerance and the control of two prolactins and their receptors in the Nile tilapia (Oreochromis niloticus) and Mozambique tilapia (O. mossambicus): A comparative study.
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DOI：
10.1016/j.ygcen.2017.06.018
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发表时间：
2018-02-01
影响因子：
2.7
通讯作者：
Seale AP
中科院分区：
文献类型：
--
作者：
Yamaguchi Y;Breves JP;Haws MC;Lerner DT;Grau EG;Seale AP
脊椎动物的渗透调节主要由神经内分泌系统控制。催乳素（PRL）是广盐性硬骨鱼在淡水中生存的关键，它能促进离子的滞留。在广盐性莫桑比克罗非鱼（Oreochromis mossambicus），垂体PRL细胞释放两个PRL亚型，PRL 188和PRL 177，在响应细胞外渗透压下降。这两种PRL通过两种PRL受体（PRLR）起作用，分别表示为PRLR1和PRLR2。本研究以尼罗罗非鱼（O. niloticus），莫桑比克罗非鱼的近亲，其对环境盐度的增加耐受性较低，以研究体内和体外急性高渗挑战对PRL和PRLR的调节。我们推测，在调节PRL和PRLR的差异的基础上的罗非鱼属奥利亚罗非鱼的耐盐性的变化。当从淡水转移到微咸水（20‰）时，尼罗罗非鱼血浆渗透压升高，循环PRL降低，尤其是PRL 177，其程度大于莫桑比克罗非鱼。在分散的PRL细胞孵育，这两个催乳素的释放是不太敏感的变化，在尼罗罗非鱼中的渗透压比莫桑比克罗非鱼。相比之下，在脑垂体和鳃prlr2基因表达的增加，在细胞外渗透压的上升更显着尼罗罗非鱼相对于其同类，在体外和体内。总之，这些结果支持的结论，两个罗非鱼同源物之间的耐盐性种间差异是绑在一起，至少部分地，这两个催乳素及其受体的渗透刺激的不同反应。
Osmoregulation in vertebrates is largely controlled by the neuroendocrine system. Prolactin (PRL) is critical for the survival of euryhaline teleosts in fresh water by promoting ion retention. In the euryhaline Mozambique tilapia (Oreochromis mossambicus), pituitary PRL cells release two PRL isoforms, PRL188 and PRL177, in response to a fall in extracellular osmolality. Both PRLs function via two PRL receptors (PRLRs) denoted PRLR1 and PRLR2. We conducted a comparative study using the Nile tilapia (O. niloticus), a close relative of Mozambique tilapia that is less tolerant to increases in environmental salinity, to investigate the regulation of PRLs and PRLRs upon acute hyperosmotic challenges in vivo and in vitro. We hypothesized that differences in the regulation of PRLs and PRLRs underlie the variation in salinity tolerance of tilapias within the genus Oreochromis. When transferred from fresh water to brackish water (20‰), Nile tilapia increased plasma osmolality and decreased circulating PRLs, especially PRL177, to a greater extent than Mozambique tilapia. In dispersed PRL cell incubations, the release of both PRLs was less sensitive to variations in medium osmolality in Nile tilapia than in Mozambique tilapia. By contrast, increases in pituitary and branchial prlr2 gene expression in response to a rise in extracellular osmolality were more pronounced in Nile tilapia relative to its congener, both in vitro and in vivo. Together, these results support the conclusion that inter-specific differences in salinity tolerance between the two tilapia congeners are tied, at least in part, to the distinct responses of both PRLs and their receptors to osmotic stimuli.
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影响因子：
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作者：
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作者：
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DOI：
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