Autologous culture method improves retention of tumors' native properties.
Autologous culture method improves retention of tumors' native properties.
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DOI：
10.1038/s41598-020-77238-0
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发表时间：
2020-11-24
影响因子：
4.6
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Tang Y;Xu Q;Yan M;Zhang Y;Zhu P;Li X;Sang L;Zhang M;Huang W;Lin L;Wu J;Xin Y;Fu J;Zhang L;Zhang S;Gu J
No current in vitro tumor model replicates a tumor’s in vivo microenvironment. A culturing technique that better preserves a tumor’s pathophysiological conditions is needed for some important clinical applications, including personalized drug-sensitivity/resistance assays. In this study, we utilized autologous serum or body fluid to build a 3D scaffold and grow a patient’s tumor. We named this technique “3D-ACM” (autologous culture method). Forty-five clinical samples from biopsies, surgically removed tumor tissues and malignant body fluids were cultured with 3D-ACM. Traditional 3D-FBS (fetal bovine serum) cultures were performed side-by-side for comparison. The results were that cells cultured in 3D-ACM rebuilt tissue-like structures, and retained their immuno-phenotypes and cytokine productions. In contrast, the 3D-FBS method promoted mesenchymal cell proliferation. In preliminary chemo drug-sensitivity assays, significantly higher mortality was always associated with FBS-cultured cells. Accordingly, 3D-ACM appears to more reliably preserve a tumor’s biological characteristics, which might improve the accuracy of drug-testing for personalized cancer treatment.
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患者衍生的异种移植模型在癌症研究中的产生和应用。
影响因子：
4.6
作者：
Jung J;Seol HS;Chang S
通讯作者：
Chang S
影响因子：
64.5
作者：
Gao D;Vela I;Sboner A;Iaquinta PJ;Karthaus WR;Gopalan A;Dowling C;Wanjala JN;Undvall EA;Arora VK;Wongvipat J;Kossai M;Ramazanoglu S;Barboza LP;Di W;Cao Z;Zhang QF;Sirota I;Ran L;MacDonald TY;Beltran H;Mosquera JM;Touijer KA;Scardino PT;Laudone VP;Curtis KR;Rathkopf DE;Morris MJ;Danila DC;Slovin SF;Solomon SB;Eastham JA;Chi P;Carver B;Rubin MA;Scher HI;Clevers H;Sawyers CL;Chen Y
通讯作者：
Chen Y
影响因子：
16.6
作者：
Majumder, Biswanath;Baraneedharan, Ulaganathan;Thiyagarajan, Saravanan;Radhakrishnan, Padhma;Narasimhan, Harikrishna;Dhandapani, Muthu;Brijwani, Nilesh;Pinto, Dency D.;Prasath, Arun;Shanthappa, Basavaraja U.;Thayakumar, Allen;Surendran, Rajagopalan;Babu, Govind K.;Shenoy, Ashok M.;Kuriakose, Moni A.;Bergthold, Guillaume;Horowitz, Peleg;Loda, Massimo;Beroukhim, Rameen;Agarwal, Shivani;Sengupta, Shiladitya;Sundaram, Mallikarjun;Majumder, Pradip K.
通讯作者：
Majumder, Pradip K.
影响因子：
3.3
作者：
Yang, C. C.;Burg, K. J. L.
通讯作者：
Burg, K. J. L.
DOI：
10.1126/science.aao2774
发表时间：
2018-02-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Vlachogiannis G;Hedayat S;Vatsiou A;Jamin Y;Fernández-Mateos J;Khan K;Lampis A;Eason K;Huntingford I;Burke R;Rata M;Koh DM;Tunariu N;Collins D;Hulkki-Wilson S;Ragulan C;Spiteri I;Moorcraft SY;Chau I;Rao S;Watkins D;Fotiadis N;Bali M;Darvish-Damavandi M;Lote H;Eltahir Z;Smyth EC;Begum R;Clarke PA;Hahne JC;Dowsett M;de Bono J;Workman P;Sadanandam A;Fassan M;Sansom OJ;Eccles S;Starling N;Braconi C;Sottoriva A;Robinson SP;Cunningham D;Valeri N
通讯作者：
Valeri N