Hematopoietic protein-1 regulates the actin membrane skeleton and membrane stability in murine erythrocytes.
Hematopoietic protein-1 regulates the actin membrane skeleton and membrane stability in murine erythrocytes.
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DOI：
10.1371/journal.pone.0054902
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Iritani BM
中科院分区：
文献类型：
--
作者：
Chan MM;Wooden JM;Tsang M;Gilligan DM;Hirenallur-S DK;Finney GL;Rynes E;Maccoss M;Ramirez JA;Park H;Iritani BM
Hematopoietic protein-1 (Hem-1) is a hematopoietic cell specific member of the WAVE (Wiskott-Aldrich syndrome verprolin-homologous protein) complex, which regulates filamentous actin (F-actin) polymerization in many cell types including immune cells. However, the roles of Hem-1 and the WAVE complex in erythrocyte biology are not known. In this study, we utilized mice lacking Hem-1 expression due to a non-coding point mutation in the Hem1 gene to show that absence of Hem-1 results in microcytic, hypochromic anemia characterized by abnormally shaped erythrocytes with aberrant F-actin foci and decreased lifespan. We find that Hem-1 and members of the associated WAVE complex are normally expressed in wildtype erythrocyte progenitors and mature erythrocytes. Using mass spectrometry and global proteomics, Coomassie staining, and immunoblotting, we find that the absence of Hem-1 results in decreased representation of essential erythrocyte membrane skeletal proteins including α- and β- spectrin, dematin, p55, adducin, ankyrin, tropomodulin 1, band 3, and band 4.1. Hem1−/− erythrocytes exhibit increased protein kinase C-dependent phosphorylation of adducin at Ser724, which targets adducin family members for dissociation from spectrin and actin, and subsequent proteolysis. Increased adducin Ser724 phosphorylation in Hem1−/− erythrocytes correlates with decreased protein expression of the regulatory subunit of protein phosphatase 2A (PP2A), which is required for PP2A-dependent dephosphorylation of PKC targets. These results reveal a novel, critical role for Hem-1 in the homeostasis of structural proteins required for formation and stability of the actin membrane skeleton in erythrocytes.
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影响因子：
7.8
作者：
Bompard, Guillaume;Caron, Emmanuelle
通讯作者：
Caron, Emmanuelle
影响因子：
4.1
作者：
Boukharov, AA;Cohen, CM
通讯作者：
Cohen, CM
影响因子：
2.3
作者：
George, Alex;Pushkaran, Suvarnamala;Kalfa, Theodosia A.
通讯作者：
Kalfa, Theodosia A.
DOI：
10.1083/jcb.200211122
发表时间：
2003-05-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Barkalow KL;Italiano JE Jr;Chou DE;Matsuoka Y;Bennett V;Hartwig JH
通讯作者：
Hartwig JH
影响因子：
20.3
作者：
An, Xiuli;Salomao, Marcela;Mohandas, Narla
通讯作者：
Mohandas, Narla