Protocol for setup and circadian analysis of inverted feeding in mice.
Protocol for setup and circadian analysis of inverted feeding in mice.
复制标题
小鼠反向喂养的设置和昼夜节律分析方案。
DOI：
10.1016/j.xpro.2021.100701
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发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Xin H;Huang R;Zhou M;Bao X;Chen J;Zeng F;Wan X;Tong S;Deng F;Li MD;Zhang Z
倒食是一种更直接地通过组织摄食节律来研究生物钟同步的范式。在这里，我们提供了一个在小鼠中进行倒立喂养和分析小鼠组织昼夜节律的方案。我们描述了倒置饲养和进行组织解剖，随后进行RNA提取和基因表达分析，最后基于R软件的昼夜节律分析。该协议可以与CircaMetDB数据库的使用相结合，进行倒灌的机理研究。有关使用和执行本协议的完整详情，请参阅。《R协议》中昼夜节律检测与比较的实践教程结合CircaMetDB资源可用于昼夜节律的机理研究。倒置喂养是一种更直接地通过组织内的摄食节律来研究昼夜节律同步的范式。在这里，我们提供了一个在小鼠中进行倒立喂养和分析小鼠组织昼夜节律的方案。我们描述了倒置饲养和进行组织解剖，随后进行RNA提取和基因表达分析，最后基于R软件的昼夜节律分析。该协议可以与CircaMetDB数据库的使用相结合，进行倒灌的机理研究。
Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tissue dissection, followed by RNA extraction and gene expression analysis, and lastly R software-based analysis of circadian rhythmicity. This protocol can be combined with the use of CircaMetDB database for mechanistic studies of inverted feeding. For complete details on the use and execution of this protocol, please refer to. Set up inverted feeding (IF) to evaluate clock synchronization by meal time Hands-on tutorial of circadian rhythmicity detection and comparison in R Protocol combined with CircaMetDB resource can be used for mechanistic study of IF Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tissue dissection, followed by RNA extraction and gene expression analysis, and lastly R software-based analysis of circadian rhythmicity. This protocol can be combined with the use of CircaMetDB database for mechanistic studies of inverted feeding.
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影响因子：
5.4
作者：
Xie X;Kukino A;Calcagno HE;Berman AM;Garner JP;Butler MP
通讯作者：
Butler MP
DOI：
10.1523/jneurosci.2191-08.2008
发表时间：
2008-11-12
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Chen R;Seo DO;Bell E;von Gall C;Lee C
通讯作者：
Lee C
多组织多组学分析揭示了反向进食夹带昼夜转录组的独特动力学。
影响因子：
5.8
作者：
Xin H;Deng F;Zhou M;Huang R;Ma X;Tian H;Tan Y;Chen X;Deng D;Shui G;Zhang Z;Li MD
通讯作者：
Li MD
影响因子：
5.8
作者：
Wu, Gang;Anafi, Ron C.;Hogenesch, John B.
通讯作者：
Hogenesch, John B.
影响因子：
5.8
作者：
Parsons, Rex;Parsons, Richard;Rawashdeh, Oliver
通讯作者：
Rawashdeh, Oliver