PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae.
PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae.
复制标题
DOI：
10.1083/jcb.147.4.845
复制
发表时间：
1999-11-15
期刊：
影响因子：
--
通讯作者：
Blumer KJ
中科院分区：
文献类型：
--
作者：
Holly SP;Blumer KJ
在酿酒酵母的细胞周期中，肌动蛋白细胞骨架和细胞表面生长被极化，介导芽的出现、芽的生长和胞质分裂。我们已经确定了p21激活激酶（PAK）家族激酶是否在酵母细胞周期的一个或几个点调节细胞和肌动蛋白极化。失活的PAK同源物Ste 20和Cla 4在细胞周期的各个点导致细胞和肌动蛋白细胞骨架极性的损失，但不是在解聚的F-肌动蛋白。PAK功能的损失在G1去极化皮质肌动蛋白细胞骨架，并阻止芽的出现，但允许各向同性的增长，并导致在septin组装缺陷，表明PAK是Rho-鸟苷三磷酸酶Cdc 42的效应。在S/G2中PAK失活导致母体和芽的去极化生长和肌动蛋白极性的丧失。有丝分裂中PAK功能的丧失导致胞质分裂的缺陷和皮层肌动蛋白细胞骨架不能附着在母芽颈上。Cla 4-绿色荧光蛋白定位于皮质肌动蛋白细胞骨架和细胞表面生长极化的位点，独立于完整的肌动蛋白细胞骨架。因此，PAK家族激酶是贯穿大部分或全部酵母细胞周期的细胞和肌动蛋白细胞骨架极性的主要调节剂。PAK家族激酶在高等生物中可能具有类似的功能。
During the cell cycle of the yeast Saccharomyces cerevisiae, the actin cytoskeleton and cell surface growth are polarized, mediating bud emergence, bud growth, and cytokinesis. We have determined whether p21-activated kinase (PAK)-family kinases regulate cell and actin polarization at one or several points during the yeast cell cycle. Inactivation of the PAK homologues Ste20 and Cla4 at various points in the cell cycle resulted in loss of cell and actin cytoskeletal polarity, but not in depolymerization of F-actin. Loss of PAK function in G1 depolarized the cortical actin cytoskeleton and blocked bud emergence, but allowed isotropic growth and led to defects in septin assembly, indicating that PAKs are effectors of the Rho–guanosine triphosphatase Cdc42. PAK inactivation in S/G2 resulted in depolarized growth of the mother and bud and a loss of actin polarity. Loss of PAK function in mitosis caused a defect in cytokinesis and a failure to polarize the cortical actin cytoskeleton to the mother-bud neck. Cla4–green fluorescent protein localized to sites where the cortical actin cytoskeleton and cell surface growth are polarized, independently of an intact actin cytoskeleton. Thus, PAK family kinases are primary regulators of cell and actin cytoskeletal polarity throughout most or all of the yeast cell cycle. PAK-family kinases in higher organisms may have similar functions.
登录
查看更多内容
DOI：
10.1083/jcb.143.3.709
发表时间：
1998-11-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carroll CW;Altman R;Schieltz D;Yates JR;Kellogg D
通讯作者：
Kellogg D
DOI：
10.1083/jcb.126.4.821
发表时间：
1994-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bretscher A;Drees B;Harsay E;Schott D;Wang T
通讯作者：
Wang T
影响因子：
11.4
作者：
Daniels, RH;Hall, PS;Bokoch, GM
通讯作者：
Bokoch, GM
影响因子：
10.5
作者：
Chen, GC;Kim, YJ;Chan, CSM
通讯作者：
Chan, CSM
影响因子：
11.4
作者：
Abo, A;Qu, J;Minden, A
通讯作者：
Minden, A