Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2).
Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2).
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弓形虫钙依赖性蛋白激酶 2 (TgCDPK2) DNA 疫苗接种后的免疫反应和保护
DOI：
10.1051/parasite/2017045
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Chen K;Wang JL;Huang SY;Yang WB;Zhu WN;Zhu XQ
弓形虫是一种细胞内人畜共患原生动物寄生虫，可能是温血动物中最广泛的寄生虫，可在全世界范围内造成严重的公共卫生问题和经济损失。 TgCDPK2 是弓形虫钙依赖性蛋白激酶家族的成员，最近被确定为弓形虫可行包囊发育的重要调节因子。在本研究中，我们评估了基于重组真核质粒 pVAX-TgCDPK2 的 DNA 疫苗接种对小鼠急性弓形虫病的保护性免疫。 BALB/c小鼠用pVAX-TgCDPK2质粒进行肌内免疫，然后用高毒力的弓形虫RH菌株感染进行攻击。通过细胞因子和血清抗体测量、淋巴细胞增殖测定、淋巴细胞流式细胞术以及攻击后小鼠的存活时间来分析针对弓形虫的特异性免疫反应和保护功效。我们的结果表明，用pVAX-TgCDPK2免疫的小鼠可以引起特殊的体液和细胞反应，IgG抗体水平较高，Th1型细胞因子IFN-γ、IL-12(p70)和CD3 + CD4 + CD8 −和CD3 + CD8 + CD4 − T细胞水平增加，并且与对照小鼠相比，其存活时间更长（14.0±2.32天）。这些结果表明 pVAX-TgCDPK2 是针对急性弓形体病的潜在候选疫苗。
Toxoplasma gondii, an intracellular zoonotic protozoan parasite, is possibly the most widespread parasite of warm-blooded animals and can cause serious public health problems and economic losses worldwide. TgCDPK2, a member of the T. gondii calcium-dependent protein kinase family, was recently identified as an essential regulator for viable cyst development in T. gondii. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic plasmid, pVAX-TgCDPK2, against acute toxoplasmosis in mice. BALB/c mice were intramuscularly immunized with pVAX-TgCDPK2 plasmid and then challenged by infection with the highly virulent RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii were analyzed by cytokine and serum antibody measurements, lymphocyte proliferation assays, flow cytometric on lymphocytes and the survival time of mice after challenge. Our results showed that mice immunized with pVAX-TgCDPK2 could elicit special humoral and cellular responses, with higher levels of IgG antibody, and increased levels of Th1-type cytokines IFN-γ, IL-12(p70), and CD3 + CD4 + CD8 − and CD3 + CD8 + CD4 − T cells, and had a prolonged survival time (14.0 ± 2.32 days) compared to control mice. These results demonstrate that pVAX-TgCDPK2 is a potential vaccine candidate against acute toxoplasmosis.
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影响因子：
11.4
作者：
Lourido, Sebastian;Tang, Keliang;Sibley, L. David
通讯作者：
Sibley, L. David
影响因子：
3.4
作者：
Morlon-Guyot J;Berry L;Chen CT;Gubbels MJ;Lebrun M;Daher W
通讯作者：
Daher W
影响因子：
4.4
作者：
Mordue, DG;Monroy, F;Sibley, LD
通讯作者：
Sibley, LD
影响因子：
5.5
作者：
Li, Zhong-Yuan;Chen, Jia;Zhu, Xing-Quan
通讯作者：
Zhu, Xing-Quan
影响因子：
64.8
作者：
通讯作者：
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