Nuclear colocalization of c-myc protein and hsp70 in cells transfected with human wild-type and mutant c-myc genes.
Nuclear colocalization of c-myc protein and hsp70 in cells transfected with human wild-type and mutant c-myc genes.
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转染人野生型和突变型 c-myc 基因的细胞中 c-myc 蛋白和 hsp70 的核共定位。
DOI：
10.1016/0014-4827(92)90012-w
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发表时间：
1992
影响因子：
3.7
通讯作者：
Thyberg,J
中科院分区：
文献类型：
--
作者：
Henriksson,M;Classon,M;Axelson,H;Klein,G;Thyberg,J
利用免疫荧光和电子显微镜，我们研究了野生型和muplyc-mycproteins在CV-1细胞中瞬时表达的定位。与我们以前的观察结果一致，野生型c-mycprotein积累在细胞核中的大的无定形球。所有测试的突变蛋白也在细胞核中积累，但产生了形态学上不同的包涵体。在用D145-262（缺失氨基酸145-262）转染的细胞中出现许多小球，而用D371-412或D414-433转染的细胞产生看起来像精细网络或像线上的珠子的结构。此外，在用野生型基因转染的一些细胞和用突变体D145-262或D414-433转染的细胞中出现颗粒状细胞质染色。由于mycprotein已被报道刺激外源hsp 70蛋白的表达，我们还检查了hsp 70在转染细胞中的细胞内分布。免疫荧光双镜显示，热休克蛋白70共分布与thec-mycprotein在不同的小球在核中的许多，但不是所有的myc-positive细胞。然而，与未转染和载体转染的细胞相比，hsp 70转录物的水平没有显著提高。同样，hsp 70蛋白的水平也没有显著变化。这些结果表明，c-myc的过度表达刺激预先存在的hsp 70从细胞质易位到细胞核，而不是影响hsp 70的表达。可以想象，这可能是细胞处理过量c-mycprotein的几种机制之一。
Using immunofluorescence and electron microscopy we have studied the localization of wild-type and mutantc-mycproteins transiently expressed in CV-1 cells. In agreement with our previous observations, wild-typec-mycprotein accumulated in large amorphous globules in the nucleus. All mutant proteins tested accumulated in the nucleus as well, but gave rise to morphologically different inclusion bodies. Many small globules appeared in cells transfected with D145–262 (deletion of amino acids 145–262), while cells transfected with D371–412 or D414–433 generated structures looking like a fine network or like beads on a string. In addition, a particulate cytoplasmic staining appeared in some cells transfected with the wild-type gene and in cells transfected with mutants D145–262 or D414–433. Since thec-mycprotein has been reported to stimulate expression of exogenous hsp70 protein, we also examined the intracellular distribution of hsp7O in the transfected cells. Double immunofluorescence microscopy revealed that hsp70 codistributed with thec-mycprotein in distinct globules in the nucleus of many but not allmyc-positive cells. However, the levels of hsp70 transcripts were not significantly raised compared to nontransfected and vector-transfected cells. Likewise, the levels of hsp70 protein did not vary significantly. These findings indicate that overexpression ofc-mycstimulates translocation of preexisting hsp70 from the cytoplasm into the nucleus, rather than influencing hsp7O expression. Conceivably, this may represent one of several mechanisms whereby the cell deals with excessive amounts ofc-mycprotein.
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影响因子：
56.9
作者：
BLACKWELL, TK;KRETZNER, L;WEINTRAUB, H
通讯作者：
WEINTRAUB, H
影响因子：
11.4
作者：
PELHAM, HRB
通讯作者：
PELHAM, HRB
c-myc 蛋白对哺乳动物基因表达的激活和抑制。
影响因子：
10.5
作者：
R. Kaddurah;J. Greene;A. Baldwin;R. Kingston
通讯作者：
R. Kingston
myc 蛋白是转录因子吗？
DOI：
--
发表时间：
1990
期刊：
Cancer cells (Cold Spring Harbor, N.Y. : 1989)
影响因子：
--
作者：
Collum,RG;Alt,FW
通讯作者：
Alt,FW
热休克：瞬时诱导反应在细胞损伤、转化和分化中的作用。
DOI：
--
发表时间：
1991
期刊：
Cancer cells
影响因子：
--
作者：
R. Morimoto
通讯作者：
R. Morimoto