Denitrification performance, biofilm formation and microbial diversity during startup of slow sand filter using powdery polycaprolactone as solid carbon source
Denitrification performance, biofilm formation and microbial diversity during startup of slow sand filter using powdery polycaprolactone as solid carbon source
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以粉状聚己内酯为固体碳源的慢砂滤池启动过程中的反硝化性能、生物膜形成及微生物多样性
DOI：
10.1016/j.jece.2021.105561
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发表时间：
2021-04
影响因子：
7.7
通讯作者：
Li Xiao-Tong
中科院分区：
文献类型：
--
作者：
Yang Lei;Guo Lin-Kai;Ren Yong-Xiang;Dou Jiang-Wei;Zhu Peng-Tao;Cui Shen;Zhang Zhi-Hao;Li Xiao-Tong
为解决慢砂滤池除氮效果不理想、启动时间长等问题，在实验室规模的慢砂滤池中加入了不同粒径（40目和200目）的粉状聚己内酯（PCL）。本研究比较了两种固体碳源在各生物膜中的释放能力和增强的反硝化性能，并通过分析生物膜的理化特性揭示了其内在机理。结果表明，pcl200的有机碳释放量为273.9 mg/L，远高于PCL40（121.2 mg/L）。由于pcl200具有较高的有机碳释放量和比表面积，有利于SSF的快速启动，并在运行初期达到理想的NO3—N（> 95%）和TN（> 80%）的去除率。此外，在pcl200系统中检测到大量的生物质和细胞外聚合物（EPS）。光谱表征进一步表明，两种SSFs的EPS荧光峰位置相似，但强度不同。其中，芳香族和色氨酸蛋白样物质是EPS的主要组分，添加PCL200后，EPS中有更多有利于微生物絮凝的功能键（C-(C， H), C˭O, O-C-O）。此外，高通量测序分析显示，在PCL200反应器中，反硝化细菌几乎占62.12%，两个SSFs中的微生物在氨基酸和碳水化合物运输和代谢方面存在显著差异。本研究结果对提高SSF处理氮污染水体的反硝化作用和稳定性具有重要意义。
To solve the problem of unsatisfactory nitrogen removal effect and long start-up in slow sand filter (SSF), the powdery polycaprolactone (PCL) with different particle sizes (40 and 200 mesh) were added to the lab-scale SSFs. This study compared the two solid carbon sources release capacity and enhanced denitrification performance in each SSF, and revealed the inner mechanism by analyzing the physical and chemical characteristics of the biofilm. The results represented that PCL200could release organic carbon up to 273.9 mg/L, which was much higher than PCL40(121.2 mg/L). Since the higher organic carbon release and specific surface area, the PCL200was conducive to the rapid start-up of the SSF and achieving a desired removal efficiency of NO3--N (> 95%) and TN (> 80%) at the early stage of operation. In addition, large amounts of biomass and extracellular polymeric substances (EPS) were detected in the PCL200system. Spectroscopic characterization further revealed EPS from two SSFs expressed similar fluorescence peak locations but different intensities. Specifically, the aromatic and tryptophan protein-like substances were the main fractions in EPS, and more functional bonds (C-(C, H), C˭O, O-C-O) conducive to microbial flocculation were observed when adding PCL200.Furthermore, the high-throughput sequencing analysis showed the denitrifying bacteria almost accounted for 62.12% in the reactor with PCL200, and microorganisms in two SSFs were significant difference in amino acid and carbohydrate transportation and metabolism. Findings from this study would be valuable for enhancement of the denitrification and stability of SSF treating nitrogen-contaminated water bodies.
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以可生物降解聚合物PCL为载体和碳源的反硝化性能及生物膜特性
影响因子：
8.8
作者：
Chu, Libing;Wang, Jianlong
通讯作者：
Wang, Jianlong
影响因子：
5.8
作者：
Srishti Verma;A. Daverey;Archana Sharma
通讯作者：
Srishti Verma;A. Daverey;Archana Sharma
DOI：
10.13227/j.hjkx.201902012
发表时间：
2019-09
期刊：
Huan jing ke xue= Huanjing kexue
影响因子：
--
作者：
C. Yi;W. Qin;Zhan Chen;X. Wen
通讯作者：
C. Yi;W. Qin;Zhan Chen;X. Wen
影响因子：
2.8
作者：
Tian-lei Qiu;Ying Xu;Min Gao;Mei-lin Han;Xuming Wang
通讯作者：
Tian-lei Qiu;Ying Xu;Min Gao;Mei-lin Han;Xuming Wang
影响因子：
15.1
作者：
Qian Zhang;F. Ji;Xiaoyi Xu
通讯作者：
Qian Zhang;F. Ji;Xiaoyi Xu