Retrospective DNA analysis of head and neck squamous cell carcinoma.
Retrospective DNA analysis of head and neck squamous cell carcinoma.
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头颈鳞状细胞癌的回顾性 DNA 分析。
DOI：
10.1001/archotol.1986.03780110035003
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Coon
中科院分区：
文献类型：
--
作者：
A. S. Kaplan;D. Caldarelli;M. Chacho;D. Bruce;J. Hutchinson;S. Conway;J. Coon
细胞 DNA 含量异常是恶性肿瘤的标志，已知发生在头颈部鳞状细胞癌中。这一发现的意义尚待阐明。将 46 个头颈部鳞状细胞癌的石蜡包埋块切片、脱蜡，并通过机械切碎和酶消化制成裸核悬浮液。细胞核用碘化丙啶染色，并通过流式细胞术获得定量 DNA 测量值。将结果与现有的预后因素进行比较：41%的标本中发现DNA非整倍体，并且与肿瘤大小和手术分期显着相关； DNA非整倍体和解剖位点之间的关系接近统计显着性。与组织学分级或是否存在宫颈转移没有相关性。
Abnormal cellular DNA content, a hallmark of malignancy, is known to occur in squamous cell carcinoma of the head and neck. The significance of this finding has yet to be elucidated. Paraffin-embedded blocks from 46 squamous cell carcinomas of the head and neck were sectioned, dewaxed, and made into a suspension of bare nuclei by mechanical mincing and enzymatic digestion. The nuclei were stained with propidium iodide, and quantitative DNA measurements were obtained with flow cytometry. The results were compared with existing prognostic factors: DNA aneuploidy was identified in 41% of the specimens and was significantly correlated with tumor size and surgical stage; the relationship between DNA aneuploidy and anatomic site approaches statistical significance. There was no correlation with histologic grade or presence or absence of cervical metastasis.
人鳞状细胞癌细胞系的 DNA 含量。
DOI：
10.1001/archotol.1985.00800110043001
发表时间：
1985
期刊：
Archives of otolaryngology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Roa,RA;Carey,TE;Passamani,PP;Greenwood,JH;Hsu,S;Ridings,EO;Schwartz,DR;Wolf,GT;Hudson,JL
通讯作者：
Hudson,JL
石蜡包埋切片中头颈癌 DNA 指数和 S 组分的流式细胞术分析：与恶性肿瘤分级的比较。
DOI：
10.1002/cyto.990060511
发表时间：
1985
期刊：
Cytometry
影响因子：
--
作者：
Johnson,TS;Williamson,KD;Cramer,MM;Peters,LJ
通讯作者：
Peters,LJ
血型抗原和倍性作为膀胱癌的预后因素。
DOI：
--
发表时间：
1986
期刊：
Monographs in pathology
影响因子：
--
作者：
Weinstein,RS;Schwartz,D;Coon,JS
通讯作者：
Coon,JS