Analysis of the subcellular distribution of RNA by fluorescence in situ hybridization.
Analysis of the subcellular distribution of RNA by fluorescence in situ hybridization.
复制标题
通过荧光原位杂交分析 RNA 的亚细胞分布。
DOI：
10.1007/978-1-4939-1369-5_10
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Nakagawa S.
中科院分区：
文献类型：
--
作者：
Shintaro Iwasaki;Hiroshi M. Sasaki;Yuriko Sakaguchi;Tsutomu Suzuki;Hisashi Tadakuma;& Yukihide Tomari;Hiroshi M Sasaki & Yukihide Tomari;Nakagawa S.
原位杂交是在亚细胞水平上研究RNA分子空间分布的一种强有力的方法，是细胞和发育生物学领域的一项基本技术。在这项技术中，目标RNA用甲醛固定在细胞中，然后与标记有修饰核苷酸的互补探针杂交，然后用免疫组织化学方法进行检测。在此，描述了在哺乳动物组织和细胞中检测RNA的常用程序，重点介绍了提高灵敏度、生产率和重复性的技术提示。
In situ hybridization is a powerful method of examining the spatial distribution of RNA molecules at the subcellular level and serves as a basic technique in the fields of cell and developmental biology. In this technique, target RNAs are fixed in cells using formaldehyde and then hybridized to complementary probes labeled with modified nucleotides that are subsequently detected by immunohistochemical methods. Here, the procedures that are commonly used for the detection of RNA in mammalian tissues and cells are described, focusing on technical tips that improve the sensitivity, productivity, and reproducibility.
影响因子：
4
作者：
Sone, Masamitsu;Hayashi, Tetsutaro;Nakagawa, Shinichi
通讯作者：
Nakagawa, Shinichi