Rad54 is not essential for any geminiviral replication mode in planta
Rad54 is not essential for any geminiviral replication mode in planta
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Rad54 对于植物中的任何双生病毒复制模式都不是必需的
DOI：
10.1007/s11103-014-0270-1
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发表时间：
2015
影响因子：
5.1
通讯作者：
Richter
中科院分区：
文献类型：
--
作者：
Richter
植物病原双生病毒的环状单链DNA通过互补链复制（CSR）、滚环复制（RCR）和重组依赖性复制（RDR）三种模式进行繁殖，需要宿主植物因子才能进行。除了必需的宿主聚合酶之外，同源重组修复途径的蛋白质可能被认为是必需的，因为双生病毒特别容易重组。其中，Rad54被认为对于印度绿豆黄花叶病毒的RCR是必需的。这种酶是一种双链 DNA 依赖性 ATP 酶和染色质重塑剂，被发现可以在体外和酿酒酵母中结合和调节病毒复制起始蛋白。与之前的报道相反，我们仔细研究了两种不同的完全感染性双生病毒在三种复制模式下对Rad54的植物需求。将大戟黄花叶病毒和Cleome叶皱病毒接种到Rad54缺陷型和野生型拟南芥植物系中，比较病毒DNA形式的发生情况。通过一维和二维琼脂糖凝胶电泳和Southern杂交显示感染时间过程中的复制中间体。实验表明，Rad54对于CSR、RCR和RDR均不是必需的，并且在全身感染过程中对病毒滴度没有显着影响。
The circular single-stranded DNA of phytopathogenic geminiviruses is propagated by three modes: complementary strand replication (CSR), rolling circle replication (RCR) and recombination-dependent replication (RDR), which need host plant factors to be carried out. In addition to necessary host polymerases, proteins of the homologous recombination repair pathway may be considered essential, since geminiviruses are particularly prone to recombination. Among several others, Rad54 was suggested to be necessary for the RCR ofMungbean yellow mosaic India virus. This enzyme is a double-stranded DNA-dependent ATPase and chromatin remodeller and was found to bind and modulate the viral replication-initiator protein in vitro and inSaccharomyces cerevisiae. In contrast to the previous report, we scrutinized therequirement of Rad54in plantafor two distinct fully infectious geminiviruses with respect to the three replication modes.Euphorbia yellow mosaic virusandCleome leaf crumple viruswere inoculated into Rad54-deficient and wildtypeArabidopsis thalianaplant lines to compare the occurrence of viral DNA forms. Replication intermediates were displayed in the time course of infection by one and two-dimensional agarose gel electrophoresis and Southern hybridization. The experiments showed that Rad54 was neither essential for CSR, RCR nor RDR, and it had no significant influence on virus titers during systemic infection.
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影响因子：
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