Two Archaeal RecJ Nucleases from Methanocaldococcus jannaschii Show Reverse Hydrolysis Polarity: Implication to Their Unique Function in Archaea.
Two Archaeal RecJ Nucleases from Methanocaldococcus jannaschii Show Reverse Hydrolysis Polarity: Implication to Their Unique Function in Archaea.
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来自詹氏甲烷球菌的两种古菌 RecJ 核酸酶显示出反向水解极性：对其在古菌中的独特功能的暗示
DOI：
10.3390/genes8090211
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发表时间：
2017-08-24
期刊：
影响因子：
3.5
通讯作者：
Liu XP
中科院分区：
文献类型：
--
作者：
Yi GS;Song Y;Wang WW;Chen JN;Deng W;Cao W;Wang FP;Xiao X;Liu XP
细菌核酸酶RecJ，存在于几乎所有的细菌物种中，特异地降解单链（ss） DNA的5 ‘到3 ’方向。除了Crenarchaea外，一些古细菌门也编码RecJ同源物。与细菌RecJ相比，古细菌RecJ的氨基酸序列和结构域组织有很大的不同。来自kodakarensis热球菌和Pyrococcus furiosus的古细菌RecJs对ssDNA具有5′→3′外切酶活性。有趣的是，在一些Euryarchaeota类中，如Methanomicrobia、Methanococci、Methanomicrobia、Methanobacteria和archaeglobi中存在不止一个RecJ。本文报道了jannaschii甲烷钙球菌（Methanocaldococcus jannaschii）中两个RecJs的生化特性，长RecJ1 （MJ0977）和短RecJ2 （MJ0831）。RecJ1是一个5 ‘→3 ’外切酶，优先于ssDNA；然而，RecJ2是一个3 ‘→5 ’外切酶，优先于ssRNA。5 ‘端磷酸促进RecJ1活性，但3 ’端磷酸抑制RecJ2核酸酶。Go-Ichi-Ni-San （GINS）复合物不与两个RecJs相互作用，也不促进它们的核酸酶活性。最后讨论了RecJ在古细菌分类中的多样性、功能和分子演化。我们的分析为保守的古细菌RecJ/真核生物Cdc45蛋白的功能和进化提供了见解。
Bacterial nuclease RecJ, which exists in almost all bacterial species, specifically degrades single-stranded (ss) DNA in the 5′ to 3′ direction. Some archaeal phyla, except Crenarchaea, also encode RecJ homologs. Compared with bacterial RecJ, archaeal RecJ exhibits a largely different amino acid sequence and domain organization. Archaeal RecJs from Thermococcus kodakarensis and Pyrococcus furiosus show 5′→3′ exonuclease activity on ssDNA. Interestingly, more than one RecJ exists in some Euryarchaeota classes, such as Methanomicrobia, Methanococci, Methanomicrobia, Methanobacteria, and Archaeoglobi. Here we report the biochemical characterization of two RecJs from Methanocaldococcus jannaschii, the long RecJ1 (MJ0977) and short RecJ2 (MJ0831) to understand their enzymatic properties. RecJ1 is a 5′→3′ exonuclease with a preference to ssDNA; however, RecJ2 is a 3′→5′ exonuclease with a preference to ssRNA. The 5′ terminal phosphate promotes RecJ1 activity, but the 3′ terminal phosphate inhibits RecJ2 nuclease. Go-Ichi-Ni-San (GINS) complex does not interact with two RecJs and does not promote their nuclease activities. Finally, we discuss the diversity, function, and molecular evolution of RecJ in archaeal taxonomy. Our analyses provide insight into the function and evolution of conserved archaeal RecJ/eukaryotic Cdc45 protein.
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