Escherichia coli-derived virus-like particles in vaccine development.
Escherichia coli-derived virus-like particles in vaccine development.
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疫苗开发中的大肠杆菌衍生病毒样颗粒
DOI：
10.1038/s41541-017-0006-8
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发表时间：
2017
期刊：
影响因子：
9.2
通讯作者：
Zhao Q
中科院分区：
文献类型：
--
作者：
Huang X;Wang X;Zhang J;Xia N;Zhao Q
基于重组病毒样颗粒的疫苗由病毒结构蛋白组成，并模拟真实的天然病毒，但缺乏病毒遗传物质。它们是预防传染病的高度安全和有效的疫苗的活性成分。从大肠杆菌到哺乳动物细胞系的几种表达系统已用于病毒样颗粒的生产。原核表达系统，特别是大肠杆菌，是生产全球使用的疫苗的优选表达宿主。Hecolin是第一个获得许可的来源于大肠杆菌的病毒样颗粒疫苗，已被证明具有良好的安全性和高效性。在这篇综述中，我们重点介绍了用于预防（针对微生物病原体的免疫）或疾病治疗（针对癌症或非传染性疾病）的基于大肠杆菌衍生病毒样颗粒的疫苗和候选疫苗。天然样的空间或更高级的结构对于病毒样颗粒的功能是必不可少的。因此，还将讨论用于分析纯化的病毒样颗粒的关键理化、生化和功能属性的工具箱。总之，大肠杆菌表达系统具有生产一系列具有自组装特性的蛋白质的巨大潜力，这些蛋白质将用作疫苗抗原，与天然病原体或疾病相关靶分子中的那些相比，保留了适当的表位。
Recombinant virus-like particle-based vaccines are composed of viral structural proteins and mimic authentic native viruses but are devoid of viral genetic materials. They are the active components in highly safe and effective vaccines for the prevention of infectious diseases. Several expression systems have been used for virus-like particle production, ranging from Escherichia coli to mammalian cell lines. The prokaryotic expression system, especially Escherichia coli, is the preferred expression host for producing vaccines for global use. Hecolin, the first licensed virus-like particle vaccine derived from Escherichia coli, has been demonstrated to possess good safety and high efficacy. In this review, we focus on Escherichia coli-derived virus-like particle based vaccines and vaccine candidates that are used for prevention (immunization against microbial pathogens) or disease treatment (directed against cancer or non-infectious diseases). The native-like spatial or higher-order structure is essential for the function of virus-like particles. Thus, the tool box for analyzing the key physicochemical, biochemical and functional attributes of purified virus-like particles will also be discussed. In summary, the Escherichia coli expression system has great potentials for producing a range of proteins with self-assembling properties to be used as vaccine antigens given the proper epitopes were preserved when compared to those in the native pathogens or disease-related target molecules.
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影响因子：
5.5
作者：
De Filette, Marina;Fiers, Walter;Saelens, Xavier
通讯作者：
Saelens, Xavier
影响因子：
4.9
作者：
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影响因子：
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作者：
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Finnefrock, Adam C.
影响因子：
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作者：
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通讯作者：
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影响因子：
7.8
作者：
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通讯作者：
Garçon N