Comparison of Protective Effects of Electroacupuncture at ST 36 and LU 5 on Pulmonary and Hypothalamic Pituitary Adrenal Axis Changes in Perinatal Nicotine-Exposed Rats
Comparison of Protective Effects of Electroacupuncture at ST 36 and LU 5 on Pulmonary and Hypothalamic Pituitary Adrenal Axis Changes in Perinatal Nicotine-Exposed Rats
复制标题
电针ST 36与LU 5对围生期尼古丁暴露大鼠肺及下丘脑垂体肾上腺轴变化的保护作用比较
DOI：
10.1155/2020/3901528
复制
发表时间：
2020-01
影响因子：
--
通讯作者：
Virender Kumar Rehan
中科院分区：
文献类型：
--
作者：
Yawen Lu;Bo Ji;Guozhen Zhao;Jian Dai;Reiko Sakurai;Yitian Liu;Qiujie Mou;Yana Xie;Qin Zhang;Shuang Xu;Virender Kumar Rehan
背景
母亲吸烟和/或暴露于环境烟草烟雾仍然是胎儿和儿童发病的重要因素，并且是世界范围内一个严重的公共卫生问题。尼古丁很容易通过胎盘，生物转化极少。
Background Maternal smoking and/or exposure to environmental tobacco smoke continue to be significant factors in fetal and childhood morbidity and are a serious public health issue worldwide. Nicotine passes through the placenta easily with minimal biotransformation, entering fetal circulation, where it results in many harmful effects on the developing offspring, especially on the developing respiratory system. Objectives Recently, in a rat model, electroacupuncture (EA) at maternal acupoints ST 36 has been shown to block perinatal nicotine-induced pulmonary damage; however, the underlying mechanism and the specificity of ST 36 acupoints for this effect are unknown. Here, we tested the hypothesis that compared with EA at ST 36, EA at LU 5 acupoints, which are on lung-specific meridian, will be equally or more effective in preventing perinatal nicotine-induced pulmonary changes. Methods Twenty-four pregnant rat dams were randomly divided into 4 groups: saline (“S”), nicotine (“N”), nicotine + ST 36 (N + ST 36), and nicotine + LU 5 (N + LU 5) groups. Nicotine (1 mg/kg, subcutaneously) and EA (at ST 36 or LU 5 acupoints, bilaterally) were administered from embryonic day 6 to postnatal day 21 once daily. The “S” group was injected saline. As needed, using ELISA, western analysis, q-RT-PCR, lung histopathology, maternal and offspring hypothalamic pituitary adrenal axes, offspring key lung developmental markers, and lung morphometry were determined. Results With nicotine exposure, alveolar count decreased, but mean linear intercept and septal thickness increased. It also led to a decrease in pulmonary function and PPARγ and an increase of β-catenin and glucocorticoid receptor expression in lung tissue and corticosterone in the serum of offspring rats. Electroacupuncture at ST 36 normalized all of these changes, whereas EA at LU 5 had no obvious effect. Conclusion Electroacupuncture applied to ST 36 acupoints provided effective protection against perinatal nicotine-induced lung changes, whereas EA applied at LU 5 acupoints was ineffective, suggesting mechanistic specificity and HPA axis' involvement in mediating EA at ST 36 acupoints' effects in mitigating perinatal nicotine-induced pulmonary phenotype. This opens the possibility that other acupoints, besides ST 36, can have similar or even more robust beneficial effects on the developing lung against the harmful effect of perinatal nicotine exposure. The approach proposed by us is simple, cheap, quick, easy to administer, and is devoid of any significant side effects.
登录
查看更多内容
影响因子：
3.7
作者：
Vesterinen HM;Morello-Frosch R;Sen S;Zeise L;Woodruff TJ
通讯作者：
Woodruff TJ
DOI：
10.1155/2016/8098561
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Jing X;Ou C;Chen H;Wang T;Xu B;Lu S;Zhu BM
通讯作者：
Zhu BM
产前尼古丁暴露与断奶后高脂饮食对不同性别大鼠子代高胆固醇血症的协同作用
影响因子：
3.1
作者：
Zhu Chunyan;Guo Yu;Luo Hanwen;Wu Yimeng;Magdalou Jacques;Chen Liaobin;Wang Hui
通讯作者：
Wang Hui
影响因子：
6.8
作者：
Mohammed MK;Shao C;Wang J;Wei Q;Wang X;Collier Z;Tang S;Liu H;Zhang F;Huang J;Guo D;Lu M;Liu F;Liu J;Ma C;Shi LL;Athiviraham A;He TC;Lee MJ
通讯作者：
Lee MJ
影响因子：
7.8
作者：
Orzabal, Marcus R.;Lunde-Young, Emilie R.;Ramadoss, Jayanth
通讯作者：
Ramadoss, Jayanth