YsxC, an essential protein in Staphylococcus aureus crucial for ribosome assembly/stability.
YsxC, an essential protein in Staphylococcus aureus crucial for ribosome assembly/stability.
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YSXC，金黄色葡萄球菌中必不可少的蛋白质对于核糖体组装/稳定性至关重要。
DOI：
10.1186/1471-2180-9-266
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发表时间：
2009-12-18
期刊：
影响因子：
4.2
通讯作者：
Foster SJ
中科院分区：
文献类型：
--
作者：
Cooper EL;García-Lara J;Foster SJ
细菌的生长和分裂需要一组核心的必需蛋白质，其中一些蛋白质的功能仍然未知。它们也是开发新抗生素的有吸引力的目标。YsxC是在真细菌中高度保守的GTP酶家族的成员，具有可能的核糖体相关功能。在这里，我们通过创造一个条件致死突变体来证明ysxC对S.金黄色。为了开始阐明YsxC功能，制备了YsxC与葡萄球菌染色体中的CBP-蛋白A标签的翻译融合物，使得能够串联亲和纯化（TAP）YsxC相互作用配偶体。这些包括核糖体蛋白S2、S10和L17，以及RNA聚合酶的β'亚基。然后，YsxC显示与核糖体共纯化作为辅助蛋白，特异性定位于50 S亚基。YsxC缺失导致成熟核糖体的存在减少，表明在S.金黄色。在本研究中，我们证明了S.金黄色葡萄球菌定位于核糖体，对核糖体的稳定性至关重要，并且显然对金黄色葡萄球菌的生命至关重要。金黄色。
Bacterial growth and division requires a core set of essential proteins, several of which are still of unknown function. They are also attractive targets for the development of new antibiotics. YsxC is a member of a family of GTPases highly conserved across eubacteria with a possible ribosome associated function. Here, we demonstrate by the creation of a conditional lethal mutant that ysxC is apparently essential for growth in S. aureus. To begin to elucidate YsxC function, a translational fusion of YsxC to the CBP-ProteinA tag in the staphylococcal chromosome was made, enabling Tandem Affinity Purification (TAP) of YsxC-interacting partners. These included the ribosomal proteins S2, S10 and L17, as well as the β' subunit of the RNA polymerase. YsxC was then shown to copurify with ribosomes as an accessory protein specifically localizing to the 50 S subunit. YsxC depletion led to a decrease in the presence of mature ribosomes, indicating a role in ribosome assembly and/or stability in S. aureus. In this study we demonstrate that YsxC of S. aureus localizes to the ribosomes, is crucial for ribosomal stability and is apparently essential for the life of S. aureus.
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