Widespread PrPSc accumulation in muscles of hamsters orally infected with scrapie
Widespread PrPSc accumulation in muscles of hamsters orally infected with scrapie
复制标题
经口感染瘙痒症的仓鼠肌肉中广泛存在 PrPSc 积累
作者：
A. Thomzig;Christine Kratzel;G. Lenz;D. Krüger;M. Beekes
羊瘙痒病、牛海绵状脑病和慢性消耗性疾病是经口传染的传染性海绵状脑病（TSE）。由于TSE病原体的人畜共患传播可能对人类健康构成风险，因此识别动物组织中的传染性宿主并将其排除在人类消费之外，已成为保护消费者的一个非常重要的问题。在这项研究中，筛选了经口感染瘙痒病的仓鼠的各种肌肉，以确定是否存在TSE感染的分子标记物PrPSc（朊病毒蛋白PrP的病理亚型）。敏感的蛋白质印迹法显示一致的PrPSc积累在骨骼肌从前肢和后肢，头部，背部和肩部，并在舌头。以前，我们的动物模型已经提供了大量的基线信息，在自然发生的和口服获得性反刍动物TSE的外周感染途径。因此，本文所述的研究结果进一步强调了彻底调查感染TSE的羊、牛、麋鹿和鹿的肌肉是否含有传染性病原体的必要性。
Scrapie, bovine spongiform encephalopathy and chronic wasting disease are orally communicable, transmissible spongiform encephalopathies (TSEs). As zoonotic transmissions of TSE agents may pose a risk to human health, the identification of reservoirs for infectivity in animal tissues and their exclusion from human consumption has become a matter of great importance for consumer protection. In this study, a variety of muscles from hamsters that were orally challenged with scrapie was screened for the presence of a molecular marker for TSE infection, PrPSc (the pathological isoform of the prion protein PrP). Sensitive western blotting revealed consistent PrPSc accumulation in skeletal muscles from forelimb and hindlimb, head, back and shoulder, and in tongue. Previously, our animal model has provided substantial baseline information about the peripheral routing of infection in naturally occurring and orally acquired ruminant TSEs. Therefore, the findings described here highlight further the necessity to investigate thoroughly whether muscles of TSE‐infected sheep, cattle, elk and deer contain infectious agents.
影响因子：
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作者：
Belay, ED;Gambetti, P;Nichols, CR
通讯作者：
Nichols, CR