Electrical stimulation induced Hsp70 response in C2C12 cells.
Electrical stimulation induced Hsp70 response in C2C12 cells.
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电刺激诱导 C2C12 细胞中的 Hsp70 反应。
DOI：
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发表时间：
2010
影响因子：
7.3
通讯作者：
J. Steinacker
中科院分区：
文献类型：
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作者：
Lei Wang;Yuefei Liu;Hong;J. Steinacker
电刺激（ES）被广泛用于实验和临床环境中，并显示对细胞应激反应的影响;然而，ES诱导效应的机制尚未完全了解。本实验观察了小鼠成肌细胞来源的C2 C12细胞在13 V电刺激下（A：12 Hz，11 min; B：12 Hz，90 min; C：100 Hz，11 min）的热休克蛋白70（Hsp 70）反应。将没有ES的对照细胞与每个刺激组平行处理。Hsp 70的表达分别在蛋白质水平上通过定量Western印迹和在mRNA水平上通过实时定量PCR测定。A组ES在mRNA水平引起适度的双相Hsp 70反应，在蛋白水平略有增加。B组Hsp 70 mRNA和蛋白水平均显著升高（P < 0.01），分别为559%和413%，并在ES后12 h仍保持较高水平。C组Hsp 70 mRNA水平在ES后4 h达到最高（14倍，P < 0.01），蛋白水平在ES后8h仅中度升高（147%，P < 0.05）。因此，ES在蛋白和mRNA水平上诱导不同的Hsp 70反应，ES的特性决定了培养细胞中Hsp 70反应的模式和时间过程。ES诱导的Hsp 70反应可能是ES多种效应的共同机制，在细胞对ES的适应性反应中起重要作用。
Electrical stimulation (ES) is widely used in experimental and clinical settings and shows effects on cellular response to stress; however; mechanisms underlying ES-induced effects are not thoroughly understood. We investigated the Hsp70 response in mouse myoblast derived C2C12 cells to ES at 13V in different groups (A: 12 Hz, 11 min; B: 12 Hz, 90 min; C: 100 Hz, 11 min) and harvested before ES and at 0h, Jh, 4h, 8h and 12h after ES, respectively. Control cells without ES were parallel treated to each stimulated group. Hsp70 expression was determined at protein level by quantitative Western-blot and at mRNA level by real-time PCR, respectively. ES in group A caused a modest biphasic Hsp70 response at mRNA level with a slight increase at protein level. In group B Hsp70 increased significantly (P < 0.01) at mRNA (559%) and protein level (413%), and remained elevated 12 h after ES. In group C the highest Hsp70 mRNA level (14-fold increase, P < 0.01) was observed at 4h after ES with only a moderate increase at protein level (147%, P < 0.05) at 8h after ES. Thus, ES induced distinct Hsp70 responses at both protein and mRNA level, and the characteristics of ES determined the pattern and time course of Hsp70 response in the cultured cells. ES induced Hsp70 response may serve as a common mechanism underlying diverse effects of ES and plays an important role in cellular adaptive response to ES.
影响因子：
3.7
作者：
Serena E;Figallo E;Tandon N;Cannizzaro C;Gerecht S;Elvassore N;Vunjak-Novakovic G
通讯作者：
Vunjak-Novakovic G
DOI：
10.1152/ajpheart.00610.2003
发表时间：
2005-04-01
影响因子：
4.8
作者：
Pedrotty, DM;Koh, J;Niklason, LE
通讯作者：
Niklason, LE
使用电刺激来增加全膝关节置换术后老年患者的股四头肌肌力。
影响因子：
3.2
作者：
Lewek,M;Stevens,J;Snyder-Mackler,L
通讯作者：
Snyder-Mackler,L