Directing cyanobacterial photosynthesis in a cytochrome c oxidase mutant using a heterologous electron sink.
Directing cyanobacterial photosynthesis in a cytochrome c oxidase mutant using a heterologous electron sink.
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DOI：
10.1093/plphys/kiac203
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发表时间：
2022-08-01
期刊：
影响因子：
7.4
通讯作者：
Bibby, Thomas S.
中科院分区：
文献类型：
--
作者：
Torrado, Alejandro;Connabeer, Hannah M.;Rottig, Annika;Pratt, Nicola;Baylay, Alison J.;Terry, Matthew J.;Moore, C. Mark;Bibby, Thomas S.
光合作用有望利用光能可持续地产生有用的产品。实现这一潜力的关键是能够合理设计光合作用，将光子产生的能量和还原剂重定向到所需的产物。细胞色素 P450 (P450) 可以催化多种反应，已被设计到多种光合生物中，其活性已被证明是光合作用依赖性的，因此充当光合作用产生的电子异源汇。此外，P450的添加可以增加宿主生物的光合作用能力。在这项研究中，我们利用蓝藻聚球藻中表达的 P450 (CYP1A1) 进一步开发了这项技术。 PCC 7002。我们表明，通过去除竞争性电子接收器（呼吸末端氧化酶细胞色素 c 氧化酶）来合理设计光合作用，可以增加 CYP1A1 的活性。我们提供的证据表明，这种增强的 CYP1A1 活性是通过光系统 I 的电子通量增加而促进的。我们还对设计的菌株进行了转录组分析，以更全面地了解细胞如何响应合理的工程。我们描述了一种复杂的反应，包括与呼吸相关的光合作用和电子转移所涉及的基因表达的变化。具体来说，CYP1A1 的表达导致其他自然电子耗散途径的表达减少。这项研究强调了在生物技术中改造光合生物的潜力，但也强调需要考虑任何合理诱导的变化对细胞代谢的更广泛影响。蓝藻的光合作用可以通过操纵天然和人工电子汇来进行。
Photosynthesis holds the promise of sustainable generation of useful products using light energy. Key to realizing this potential is the ability to rationally design photosynthesis to redirect energy and reductant derived from photons to desired products. Cytochrome P450s (P450s), which catalyze a broad array of reactions, have been engineered into a variety of photosynthetic organisms, where their activity has been shown to be photosynthesis-dependent, thus acting as heterologous sinks of electrons derived from photosynthesis. Furthermore, the addition of P450s can increase the photosynthetic capacity of the host organism. In this study, we developed this technology further using a P450 (CYP1A1) expressed in the cyanobacterium Synechococcus sp. PCC 7002. We show that rationally engineering photosynthesis by the removal of a competing electron sink, the respiratory terminal oxidase cytochrome c oxidase, increased the activity of CYP1A1. We provide evidence that this enhanced CYP1A1 activity was facilitated via an increase in the flux of electrons through Photosystem I. We also conducted a transcriptomic analysis on the designed strains to gain a more holistic understanding of how the cell responds to rational engineering. We describe a complex response including changes in expression of genes involved in photosynthesis and electron transfer linked to respiration. Specifically, the expression of CYP1A1 resulted in the reduction in expression of other natural electron dissipation pathways. This study emphasizes the potential for engineering photosynthetic organisms in biotechnology but also highlights the need to consider the broader impacts on cellular metabolism of any rationally induced changes. Photosynthesis in cyanobacteria can be engineered through the manipulation of both natural and artificial electron sinks.
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影响因子：
48
作者：
Huber W;Carey VJ;Gentleman R;Anders S;Carlson M;Carvalho BS;Bravo HC;Davis S;Gatto L;Girke T;Gottardo R;Hahne F;Hansen KD;Irizarry RA;Lawrence M;Love MI;MacDonald J;Obenchain V;Oleś AK;Pagès H;Reyes A;Shannon P;Smyth GK;Tenenbaum D;Waldron L;Morgan M
通讯作者：
Morgan M
影响因子：
6.1
作者：
Kawahigashi, H;Hirose, S;Ohkawa, Y
通讯作者：
Ohkawa, Y
DOI：
10.1073/pnas.1221194110
发表时间：
2013-03-05
影响因子：
11.1
作者：
Allahverdiyeva, Yagut;Mustila, Henna;Aro, Eva-Mari
通讯作者：
Aro, Eva-Mari
影响因子：
7.4
作者：
Ermakova, Maria;Huokko, Tuomas;Allahverdiyeva, Yagut
通讯作者：
Allahverdiyeva, Yagut
影响因子：
5.6
作者：
Gnanasekaran T;Vavitsas K;Andersen-Ranberg J;Nielsen AZ;Olsen CE;Hamberger B;Jensen PE
通讯作者：
Jensen PE