Block copolymer nanoparticles-based fluorescent sensor for ultrasensitive detection of tyrosinase activity and inhibitor
Block copolymer nanoparticles-based fluorescent sensor for ultrasensitive detection of tyrosinase activity and inhibitor
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基于嵌段共聚物纳米颗粒的荧光传感器，用于超灵敏检测酪氨酸酶活性和抑制剂
DOI：
10.1016/j.snb.2019.126935
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发表时间：
2019-11
期刊：
影响因子：
--
通讯作者：
Chen Jian
中科院分区：
文献类型：
--
作者：
Liu Ying;Zhang Chong-Hua;Zhang Peisheng;Wang Hong;Liu Jin-Wen;Wang Shenglan;Zeng Rongjin;Chen Shu;Chen Jian
开发一种简单、快速、无标签、灵敏的酪氨酸酶活性检测及抑制剂筛选方法在生物医学诊断和化妆品行业具有重要的应用价值。然而，许多最近报道的荧光分析可能遭受光照射不稳定，水溶性差，和复杂的修饰。本文研究了一种基于荧光嵌段共聚物纳米颗粒（BCNs）的新型荧光生物传感器策略，该策略能够灵敏、无标记、快速地筛选酪氨酸酶活性以及相应的抑制剂。用两亲性嵌段共聚物和荧光共轭聚合物的混合物通过共沉淀法制备了BCNs，具有亮度高、光稳定性好和生物相容性好等优点，使得该生物传感器具有高灵敏度和重复性好等优点。该方法对酪氨酸酶（TRY）活性检测具有较高的灵敏度和选择性，检测限为1.5 ng/mL，在酪氨酸酶抑制剂筛选中具有一定的潜力。令人满意的回收率为复杂样品分析提供了巨大的潜力。因此，这一报道可能为生物传感器的开发提供了一个新的平台。
It is of great value to develop a simple, rapid, label-free and sensitive strategy for tyrosinase activity detection and corresponding inhibitor screening in both biomedical diagnosis and cosmetic industry. However, many recently reported fluorescent assays may suffer from instability to light irradiation, poor water solubility, and complex modification. Herein, a novel fluorescent biosensor strategy has been developed capable of sensitive, label-free and rapid screening of tyrosinase activity as well as corresponding inhibitors based on fluorescent block copolymer nanoparticles (BCNs). The reported BCNs were prepared by coprecipitation assay using the mixture of amphiphilic block copolymer and fluorescent conjugated polymer, which show many advantages including high brightness, excellent photostability and biocompatibility, enabling the biosensor with high sensitivity and good reproducibility in a single-step operation. The reported assay exhibited high sensitivity and selectivity with a detection limit of 1.5 ng/mL for tyrosinase (TRY) activity detection and hold potential in its inhibitor screening. The satisfying recoveries offered great potential for complicated sample analysis. Hence, this reported assay may provide a novel platform for biosensor development.
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生物成像近红外荧光探针开发的一般策略。
DOI：
10.1002/anie.201710688
发表时间：
2017-12-22
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Chen W;Xu S;Day JJ;Wang D;Xian M
通讯作者：
Xian M
影响因子：
4.8
作者：
Isabella Tessari;M. Bisaglia;F. Valle;B. Samorì;E. Bergantino;S. Mammi;L. Bubacco
通讯作者：
Isabella Tessari;M. Bisaglia;F. Valle;B. Samorì;E. Bergantino;S. Mammi;L. Bubacco
影响因子：
4.9
作者：
Xiaohua Li;Wen Shi;Suming Chen;J. Jia;Huimin Ma;O. Wolfbeis
通讯作者：
Xiaohua Li;Wen Shi;Suming Chen;J. Jia;Huimin Ma;O. Wolfbeis
影响因子：
7.4
作者：
Teng, Ye;Jia, Xiaofang;Wang, Erkang
通讯作者：
Wang, Erkang
影响因子：
4.9
作者：
Qingling Xu;Juyoung Yoon
通讯作者：
Qingling Xu;Juyoung Yoon