Effects of interferon gamma and major histocompatibility complex-encoded subunits on peptidase activities of human multicatalytic proteases.
Effects of interferon gamma and major histocompatibility complex-encoded subunits on peptidase activities of human multicatalytic proteases.
复制标题
干扰素γ和主要组织相容性复合物编码亚基对人类多催化蛋白酶肽酶活性的影响。
DOI：
10.1073/pnas.92.2.584
复制
发表时间：
1995
影响因子：
11.1
通讯作者：
Rechsteiner,M
中科院分区：
文献类型：
--
作者：
Ustrell,V;Pratt,G;Rechsteiner,M
我们已经研究了几种肽酶活性的人多催化蛋白酶（MCP）纯化的淋巴母细胞系721.45和缺失突变衍生物，721.174，缺乏MCP亚基编码的主要组织相容性复合体（MHC）II类区域。野生型淋巴母细胞MCP水解一种特定的肽，戊二酰-Gly-Gly-Phe-4-甲基香豆酰-7-酰胺（-MCA），比突变酶快几倍，这表明MHC编码的亚基可能提供这种活性。与最近的一份报告相反[Drivel，J.，布朗，M。G.，Finley，D.和Monaco，JJ.（1993）Nature（伦敦）365，262 - 264]，我们没有检测到与淋巴母细胞MCP相关的显著的氨肽酶。我们的结果也与Gaczynska等人[Gaczynska，M.，洛克，K。L. & Goldberg，A L.（1993）Nature（伦敦）365，264 - 267]，他报道了γ干扰素（IFN-γ）改变淋巴母细胞MCP的肽酶活性。我们发现，IFN-γ并没有产生显着差异纯化MCP的肽酶活性。此外，我们测量的Vmax和Km琥珀酰-亮氨酸-亮氨酸-缬氨酸-酪氨酸-MCA水解不同600倍和15倍，分别从Gaczynska等报道的平衡，在这里提出的研究结果不支持的想法，IFN-γ诱导纯化MCP的肽酶活性的重大变化。
We have examined several peptidase activities of human multicatalytic protease (MCP) purified from the lymphoblastoid cell line 721.45 and a deletion mutant derivative, 721.174, lacking MCP subunits encoded in the major histocompatibility complex (MHC) class II region. Wild-type lymphoblast MCP hydrolyzed a specific peptide, glutaryl-Gly-Gly-Phe-4-methylcoumaryl-7-amide (-MCA), several times faster than the mutant enzyme did, suggesting that MHC-encoded subunits may provide this activity. Contrary to a recent report [Driscoll, J., Brown, M. G., Finley, D. & Monaco, J J. (1993) Nature (London) 365, 262-264], we did not detect significant aminopeptidase associated with lymphoblast MCPs. Our results also differ markedly from those of Gaczynska et al. [Gaczynska, M., Rock, K. L. & Goldberg, A L. (1993) Nature (London) 365, 264-267], who reported that gamma interferon (IFN-gamma) alters the peptidase activities of lymphoblast MCPs. We found that IFN-gamma did not produce significant differences in the peptidase activities of purified MCPs. Moreover, our measurements of Vmax and Km for succinyl-Leu-Leu-Val-Tyr-MCA hydrolysis differ 600-fold and 15-fold, respectively, from those reported by Gaczynska et al. On balance, the findings presented here do not support the idea that IFN-gamma induces major changes in the peptidase activity of purified MCPs.
影响因子：
64.8
作者：
KELLY, A;POWIS, SH;TROWSDALE, J
通讯作者：
TROWSDALE, J