Incompatible insect technique: incompatible males from a Ceratitis capitata genetic sexing strain
Incompatible insect technique: incompatible males from a Ceratitis capitata genetic sexing strain
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不亲和昆虫技术：来自头状实蝇遗传性别鉴定品系的不亲和雄性
DOI：
10.1111/j.1570-7458.2009.00886.x
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发表时间：
2009
影响因子：
1.9
通讯作者：
K. Bourtzis
中科院分区：
文献类型：
--
作者：
S. Zabalou;A. Apostolaki;I. Livadaras;G. Franz;A. Robinson;C. Savakis;K. Bourtzis
沃尔巴克氏体是必需的细胞内和母系遗传细菌，通过诱导雄性杀伤、雌性化、孤雌生殖以及最常见的单向和双向细胞质不相容 (CI) 来感染自然节肢动物群体并传播。细胞质不相容性可用于控制害虫的自然种群，其方式类似于昆虫不育技术（SIT），即通过不相容昆虫技术（IIT）。为了成功地将 IIT（基于单向 CI 方法）应用于目标物种，必须只释放雄性，因为释放雌性会导致释放的雄性和释放的雌性之间的可育交配，并建立携带沃尔巴克氏体的野外种群。在本研究中，我们描述了地中海果蝇 VIENNA 8 遗传性别鉴定品系的 Wolbachia 感染品系，Ceratitis headata (Wiedemann)（双翅目：实蝇科），其携带选择性标记温度敏感致死 (tsl)。我们表明，（1）即使在仅雄性生产所需的温度处理之后，转移的沃尔巴克氏体也会诱导高水平的 CI，（2）感染沃尔巴克氏体的遗传性别鉴定 C. headata 系可用于类似于 SIT 的笼子种群抑制实验。我们还讨论了 IIT 和 SIT 的比较结果，研究是否可以将辐照和细胞质因子结合起来，以开发新的害虫控制策略。
Wolbachia are obligatory intracellular and maternally inherited bacteria that infect and spread through natural arthropod populations by inducing male‐killing, feminization, parthenogenesis, and, most commonly, unidirectional and bidirectional cytoplasmic incompatibility (CI). Cytoplasmic incompatibility can be used to control natural populations of insect pests, in a way analogous to the Sterile Insect Technique (SIT), namely through the Incompatible Insect Technique (IIT). For the successful application of the IIT (based on a unidirectional CI approach) against a target species, it is essential that only males are released, as the release of females would lead to fertile matings between the released males and the released females and the establishment of a Wolbachia‐carrying field population. In the present study, we describe a Wolbachia‐infected line of the VIENNA 8 genetic sexing strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), that carries the selectable marker temperature sensitive lethal (tsl). We show that (1) transferred Wolbachia induce high levels of CI even after the temperature treatment required for the male‐only production, and (2) the Wolbachia‐infected genetic sexing C. capitata line can be used in cage population suppression experiments analogous to the SIT. We also discuss our results in a comparison between IIT and SIT, investigating whether irradiation and cytoplasmic factors can be combined toward the development of novel strategies for insect pest control.
影响因子：
3.3
作者：
J. Rasgon;T. Scott
通讯作者：
J. Rasgon;T. Scott
沃尔巴克氏体感染和热休克对模拟果蝇精子产生的抵消作用：生育力、生育力和辅助腺蛋白的分析。
影响因子：
3.3
作者：
Snook,RR;Cleland,SY;Wolfner,MF;Karr,TL
通讯作者：
Karr,TL