Continuous measurement of surface electrical potentials from transplanted cardiomyocyte tissue derived from human-induced pluripotent stem cells under physiological conditions in vivo
Continuous measurement of surface electrical potentials from transplanted cardiomyocyte tissue derived from human-induced pluripotent stem cells under physiological conditions in vivo
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在体内生理条件下连续测量源自人诱导多能干细胞的移植心肌细胞组织的表面电位
DOI：
10.1007/s00380-021-01824-z
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发表时间：
2021
期刊：
影响因子：
1.5
通讯作者：
Tatsuya Shimizu
中科院分区：
文献类型：
--
作者：
Hiroshi Goto;Hyoe Komae;Hidekazu Sekine;Jun Homma;Sunghoon Lee;Tomoyuki Yokota;Katsuhisa Matsuura;Takao Someya;Minoru Ono;Tatsuya Shimizu
记录移植后生物工程心脏组织的电位将有助于监测组织的成熟和检测心律失常等不良事件。然而，少数研究报道了在生理条件下测量体内心肌组织电位。在这项研究中，人诱导的多能干细胞衍生的心肌细胞（hiPSCM）片层堆叠和异位移植到皮下组织的大鼠体内培养。移植后三个月，将柔性纳米网传感器植入到hiPSCM组织上，以记录其在生理条件下的表面电位，即，而不需要可能不利地影响心肌细胞功能的麻醉剂。纳米网传感器能够记录非镇静、走动动物的电位长达48小时。当与麻醉动物中用常规针电极进行的记录相比时，用纳米网传感器获得的波形显示波形间隔和波形持续时间的分散性较小。然而，纳米网传感器的波形振幅倾向于显示出比针电极更大的分散，这可能是由于响应于传感器的手术植入而由动物的运动或局部组织变化产生的运动伪影。本研究中使用的可植入纳米网传感器可能用于在生理条件下长期监测体内生物工程心肌组织。
Recording the electrical potentials of bioengineered cardiac tissue after transplantation would help to monitor the maturation of the tissue and detect adverse events such as arrhythmia. However, a few studies have reported the measurement of myocardial tissue potentials in vivo under physiological conditions. In this study, human-induced pluripotent stem cell-derived cardiomyocyte (hiPSCM) sheets were stacked and ectopically transplanted into the subcutaneous tissue of rats for culture in vivo. Three months after transplantation, a flexible nanomesh sensor was implanted onto the hiPSCM tissue to record its surface electrical potentials under physiological conditions, i.e., without the need for anesthetic agents that might adversely affect cardiomyocyte function. The nanomesh sensor was able to record electrical potentials in non-sedated, ambulating animals for up to 48 h. When compared with recordings made with conventional needle electrodes in anesthetized animals, the waveforms obtained with the nanomesh sensor showed less dispersion of waveform interval and waveform duration. However, waveform amplitude tended to show greater dispersion for the nanomesh sensor than for the needle electrodes, possibly due to motion artifacts produced by movements of the animal or local tissue changes in response to surgical implantation of the sensor. The implantable nanomesh sensor utilized in this study potentially could be used for long-term monitoring of bioengineered myocardial tissue in vivo under physiological conditions.
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