A RanBP2-type zinc finger protein functions in intron splicing in Arabidopsis mitochondria and is involved in the biogenesis of respiratory complex I.
A RanBP2-type zinc finger protein functions in intron splicing in Arabidopsis mitochondria and is involved in the biogenesis of respiratory complex I.
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DOI：
10.1093/nar/gkab066
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发表时间：
2021-04-06
影响因子：
14.9
通讯作者：
Hanson MR
中科院分区：
文献类型：
--
作者：
Bentolila S;Gipson AB;Kehl AJ;Hamm LN;Hayes ML;Mulligan RM;Hanson MR
RanBP 2锌指（Znf）结构域是介导蛋白质相互作用和RNA结合的普遍结构域。在拟南芥中，已知或预测四个含有RanBP 2 Znf的蛋白质的进化枝，称为细胞器锌（OZ）指家族，被靶向线粒体或质体。以前我们报道过OZ 1是叶绿体中14个位点的编辑所必需的。我们现在已经研究了OZ 2的功能，其无效突变是胚胎致死的。我们通过在种子特异性ABSCISIC ACID-INSENSITIVE 3（ABI 3）启动子的控制下表达野生型OZ 2来拯救无效突变体。挽救的突变体植物表现出严重延迟的发展和独特的形态表型。遗传和生化分析表明，OZ 2促进剪接的几个线粒体nad基因和rps 3的转录本。nad转录本的剪接缺陷导致复合体I的不稳定，从而影响oz 2突变体的呼吸能力，开启替代呼吸途径，影响植物的发育。蛋白质-蛋白质相互作用分析表明OZ 2与几种已知的线粒体剪接因子结合，靶向相同的剪接事件。这些发现扩展了RanBP 2锌指结构域在核剪接中的已知功能库，包括植物细胞器剪接。
The RanBP2 zinc finger (Znf) domain is a prevalent domain that mediates protein interaction and RNA binding. In Arabidopsis, a clade of four RanBP2 Znf-containing proteins, named the Organelle Zinc (OZ) finger family, are known or predicted to be targeted to either the mitochondria or the plastids. Previously we reported that OZ1 is absolutely required for the editing of 14 sites in chloroplasts. We now have investigated the function of OZ2, whose null mutation is embryo lethal. We rescued the null mutant by expressing wild-type OZ2 under the control of the seed-specific ABSCISIC ACID-INSENSITIVE3 (ABI3) promoter. Rescued mutant plants exhibit severely delayed development and a distinctive morphological phenotype. Genetic and biochemical analyses demonstrated that OZ2 promotes the splicing of transcripts of several mitochondrial nad genes and rps3. The splicing defect of nad transcripts results in the destabilization of complex I, which in turn affects the respiratory ability of oz2 mutants, turning on the alternative respiratory pathway, and impacting the plant development. Protein-protein interaction assays demonstrated binding of OZ2 to several known mitochondrial splicing factors targeting the same splicing events. These findings extend the known functional repertoire of the RanBP2 zinc finger domain in nuclear splicing to include plant organelle splicing.
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影响因子：
3.3
作者：
Bentolila, Stephane;Elliott, Leah E.;Hanson, Maureen R.
通讯作者：
Hanson, Maureen R.
影响因子：
7.2
作者：
Brangeon, J;Sabar, M;De Paepe, R
通讯作者：
De Paepe, R
影响因子：
7.4
作者：
Bentolila, Stephane;Knight, Walter;Hanson, Maureen
通讯作者：
Hanson, Maureen
五肽重复MTSF1蛋白在拟南芥线粒体中稳定NAD4 mRNA。
影响因子：
14.9
作者：
Haïli N;Arnal N;Quadrado M;Amiar S;Tcherkez G;Dahan J;Briozzo P;Colas des Francs-Small C;Vrielynck N;Mireau H
通讯作者：
Mireau H
影响因子：
4.8
作者：
Higa, Meda M.;Alam, Steven L.;Ullman, Katharine S.
通讯作者：
Ullman, Katharine S.