Transposon Ac/Ds-induced chromosomal rearrangements at the rice OsRLG5 locus.
Transposon Ac/Ds-induced chromosomal rearrangements at the rice OsRLG5 locus.
复制标题
DOI：
10.1093/nar/gkr718
复制
发表时间：
2011-12
影响因子：
14.9
通讯作者：
Han CD
中科院分区：
文献类型：
--
作者：
Xuan YH;Piao HL;Je BI;Park SJ;Park SH;Huang J;Zhang JB;Peterson T;Han CD
以往的研究表明，对紧密连接的Ac/Ds转座元件可以诱导植物基因组中的各种染色体重排。为了研究水稻的染色体重排，我们分离了一个在OsRLG5 （Oryza sativa Receptor like kinase Gene 5）中含有两个反向Ds插入的品系（OsRLG5-161）。在由OsRLG5-161杂合种子再生的约300株植株中，有107株存在不同大小的重排，包括缺失、重复和倒置。大多数重排是由先前确定的替代转位机制引起的。此外，我们还发现了一类新的重排，在同一染色体上包含并置反转和缺失。我们提出这些新的等位基因是由以前未报道的一种替代转位反应产生的，涉及位于同一染色单体上的两个反向定向的Ds元素的5 ‘和3 ’端。最后，11%的重排包含由OsRLG5-161中两个倒d元素之间的同源重组引起的倒置。高频率遗传和大量重排表明，水稻再生系统导致了转位活性的爆发，并放松了通常限制单个Ds末端转位能力的控制。总之，这些结果显示了Ac/Ds系统在植物染色体工程中的巨大潜力。
Previous studies have shown that pairs of closely-linked Ac/Ds transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line (OsRLG5-161) that contains two inversely-oriented Ds insertions in OsRLG5 (Oryza sativa Receptor like kinase Gene 5). Among approximately 300 plants regenerated from OsRLG5-161 heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented Ds elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted Ds elements in OsRLG5-161. The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual Ds termini. Together, these results demonstrate a greatly enlarged potential of the Ac/Ds system for plant chromosome engineering.
登录
查看更多内容
DOI：
10.1073/pnas.93.15.7783
发表时间：
1996-07-23
影响因子：
11.1
作者：
Hirochika, H;Sugimoto, K;Kanda, M
通讯作者：
Kanda, M
影响因子：
11.6
作者：
Wang, LH;Heinlein, M;Kunze, R
通讯作者：
Kunze, R
影响因子：
5.1
作者：
Krishnaswamy, Lakshminarasimhan;Zhang, Jianbo;Peterson, Thomas
通讯作者：
Peterson, Thomas
影响因子：
7.2
作者：
Chin, HG;Choe, MS;Han, CD
通讯作者：
Han, CD
影响因子：
7.2
作者：
Kim, CM;Piao, HL;Han, CD
通讯作者：
Han, CD