Molecular evolutionary analysis of the Alfin-like protein family in Arabidopsis lyrata, Arabidopsis thaliana, and Thellungiella halophila.
Molecular evolutionary analysis of the Alfin-like protein family in Arabidopsis lyrata, Arabidopsis thaliana, and Thellungiella halophila.
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DOI：
10.1371/journal.pone.0066838
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cannon CH
中科院分区：
文献类型：
--
作者：
Song Y;Gao J;Yang F;Kua CS;Liu J;Cannon CH
在以前的研究中，Alfin 1基因，一种转录因子，主要通过改变根中的基因表达水平来增强苜蓿的耐盐性。在这里，我们研究了两种拟南芥中Alfin样（AL）蛋白的分子进化。lyrata和A. thaliana）和一种耐盐的近亲盐芥Thellungiella halophila。这些AL样蛋白可以分为4组，并且由于三个物种的共同祖先中的基因复制事件，两个已知的DUF 3594和PHD-finger结构域在每组基因中共同进化，而仅在T.喜盐植物为了探讨自然选择是否在AL基因的进化中起作用，我们计算了同义替换率（dn/ds）和密码子使用统计，发现正选择作用于四个分支，并且在T.嗜盐菌A. lyrata或A. thaliana.不同的是，只有AL 7分支在PHD-指结构域上处于正选择下，并且当在七个簇之间评估密码子偏好性时，分支上的三个成员显示出最小的差异。基于转基因过量表达株系和T-DNA插入突变体的功能分析表明，盐胁迫诱导的AtAL 7在盐胁迫下对拟南芥的耐盐性可能起负作用。thaliana的基因，表明AL基因家族成员发生了适应性进化。
In previous studies, the Alfin1 gene, a transcription factor, enhanced salt tolerance in alfalfa, primarily through altering gene expression levels in the root. Here, we examined the molecular evolution of the Alfin-like (AL) proteins in two Arabidopsis species (A. lyrata and A. thaliana) and a salt-tolerant close relative Thellungiella halophila. These AL-like proteins could be divided into four groups and the two known DUF3594 and PHD-finger domains had co-evolved within each group of genes, irrespective of species, due to gene duplication events in the common ancestor of all three species while gene loss was observed only in T. halophila. To detect whether natural selection acted in the evolution of AL genes, we calculated synonymous substitution ratios (dn/ds) and codon usage statistics, finding positive selection operated on four branches and significant differences in biased codon usage in the AL family between T. halophila and A. lyrata or A. thaliana. Distinctively, only the AL7 branch was under positive selection on the PHD-finger domain and the three members on the branch showed the smallest difference when codon bias was evaluated among the seven clusters. Functional analysis based on transgenic overexpression lines and T-DNA insertion mutants indicated that salt-stress-induced AtAL7 could play a negative role in salt tolerance of A. thaliana, suggesting that adaptive evolution occurred in the members of AL gene family.
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影响因子：
7.2
作者：
Lee, Woo Yong;Lee, Daeyoup;Kwon, Chang Seob
通讯作者：
Kwon, Chang Seob
两个层面的进化：基因和形态。
影响因子：
9.8
作者：
Carroll, SB
通讯作者：
Carroll, SB
影响因子：
3.3
作者：
Chang, SM;Lu, YQ;Rausher, MD
通讯作者：
Rausher, MD
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F