Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors.
Immobilization Strategies for Enhancing Sensitivity of Electrochemical Aptamer-Based Sensors.
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DOI：
10.1021/acsami.0c20707
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发表时间：
2021-03-03
影响因子：
9.5
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Liu Y;Canoura J;Alkhamis O;Xiao Y
基于电化学适配体 (E-AB) 的传感器是一种多功能传感平台，可以在复杂基质中实现快速、稳健的目标检测。然而，这些传感器有限的灵敏度阻碍了它们从概念验证到商业产品的转化。表面结合的适体必须有足够的空间来结合靶标并随后折叠以进行信号转导。我们假设使用传统方法制造的电极会导致传感表面上只有一小部分适体适当间隔以主动响应目标。作为替代方案，我们提出了一种新颖的适体固定方法，该方法有利于在微尺度上适体之间有足够的间距，以实现最佳的靶标结合、折叠和信号转导。我们首先证明，将适体以其目标结合、折叠状态固定在金电极表面上可产生单层适体，与传统制备的 E-AB 传感器相比，它具有更高的灵敏度和更高的信噪比。我们还表明，在低离子强度条件下进行适体固定而不是传统的高离子强度缓冲液可以大大提高 E-AB 传感器的性能。我们成功地用三种不同的小分子结合适体测试了我们的方法，证明了其普遍性。基于这些结果，我们相信我们的电极制造方法将加速高性能传感器的开发，其灵敏度可满足实际分析应用的需要。
Electrochemical aptamer-based (E-AB) sensors are a versatile sensing platform that can achieve rapid and robust target detection in complex matrices. However, the limited sensitivity of these sensors has impeded their translation from proof-of-concept to commercial products. Surface-bound aptamers must be sufficiently spaced to bind targets and subsequently fold for signal transduction. We hypothesized that electrodes fabricated using conventional methods result in sensing surfaces where only a fraction of aptamers are appropriately spaced to actively respond to the target. As an alternative, we presented a novel aptamer immobilization approach that favors sufficient spacing between aptamers at the microscale to achieve optimal target binding, folding, and signal transduction. We first demonstrated that immobilizing aptamers in their target-bound, folded state on gold electrode surfaces yields an aptamer monolayer that supports greater sensitivity and higher signal-to-noise ratio than traditionally prepared E-AB sensors. We also showed that performing aptamer immobilization under low ionic strength conditions rather than conventional high ionic strength buffer greatly improves E-AB sensor performance. We successfully tested our approach with three different small-molecule-binding aptamers, demonstrating its generalizability. On the basis of these results, we believe our electrode fabrication approach will accelerate development of high-performance sensors with the sensitivity required for real-world analytical applications.
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影响因子：
7.4
作者：
Liu Y;Yu H;Alkhamis O;Moliver J;Xiao Y
通讯作者：
Xiao Y
使用核酸外切酶消化加速 SELEX 后适体工程。
影响因子：
15
作者：
Canoura J;Yu H;Alkhamis O;Roncancio D;Farhana R;Xiao Y
通讯作者：
Xiao Y
通过核酸酶定向截断将结构转换功能引入小分子结合适体
影响因子：
14.9
作者：
Wang Z;Yu H;Canoura J;Liu Y;Alkhamis O;Fu F;Xiao Y
通讯作者：
Xiao Y
DOI：
10.1073/pnas.1119057109
发表时间：
2012-01-17
影响因子：
11.1
作者：
Chen, Huimin;Meisburger, Steve P.;Pollack, Lois
通讯作者：
Pollack, Lois
影响因子：
2.5
作者：
Jones, Alan Wayne;Holmgren, Anita
通讯作者：
Holmgren, Anita