Intraparenchymal Neural Stem/Progenitor Cell Transplantation for Ischemic Stroke Animals: A Meta-Analysis and Systematic Review.
Intraparenchymal Neural Stem/Progenitor Cell Transplantation for Ischemic Stroke Animals: A Meta-Analysis and Systematic Review.
复制标题
缺血性中风动物的实质内神经干/祖细胞移植：荟萃分析和系统评价
DOI：
10.1155/2018/4826407
复制
发表时间：
2018
影响因子：
4.3
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Huang H;Qian K;Han X;Li X;Zheng Y;Chen Z;Huang X;Chen H
脑实质内移植神经干/祖细胞（NSPCs）在缺血性卒中动物模型中已被广泛研究。然而，报告的治疗疗效在研究中不一致。为了评估这种情况，检索了PubMed、Embase和Web of Science数据库中使用NSPC脑实质内移植治疗缺血性卒中动物的临床前研究。提取研究质量评分、神经行为（mNSS、旋转棒试验和圆柱体试验）和组织学（梗死体积）结局、细胞治疗相关严重不良事件和相关细胞机制的数据进行荟萃分析和系统评价。根据我们的标准，共纳入了62项研究，包括73个治疗组，10项研究的平均质量评分为5.10。在这些研究中，几乎一半的研究声称没有肿瘤发生的不良事件。神经行为和组织学评估的最终合并效应量较大（mNSS为1.27，旋转杆试验为1.63，圆柱试验为0.71，梗死体积减少为1.11）。进一步分析发现，脑卒中后给药时间、NSPC供体种类、移植免疫原性与梗死体积缩小程度密切相关。递送到脑实质中的NSPC剂量也与圆筒试验的效果呈负相关。有趣的是，内源性细胞凋亡抑制和轴突再生在实质内NSPC移植的细胞机制中起着最关键的作用。这些结果表明，脑实质内移植NSPC是有益的神经行为和组织学的改善，是相对安全的缺血性中风动物。因此，脑实质内NSPC移植是治疗脑卒中患者的一种有前途的方法。
Intraparenchymal transplantation of neural stem/progenitor cells (NSPCs) has been extensively investigated in animal models of ischemic stroke. However, the reported therapeutic efficacy was inconsistent among studies. To evaluate this situation, PubMed, Embase, and Web of Science databases were searched for preclinical studies using NSPC intraparenchymal transplantation in ischemic stroke animals. Data of study quality score, neurobehavioral (mNSS, rotarod test, and cylinder test) and histological (infarct volume) outcomes, cell therapy-related serious adverse events, and related cellular mechanisms were extracted for meta-analysis and systematic review. A total of 62 studies containing 73 treatment arms were included according to our criterion, with a mean quality score of 5.10 in 10. Among these studies, almost half of the studies claimed no adverse events of tumorigenesis. The finally pooled effect sizes for neurobehavioral and histological assessments were large (1.27 for mNSS, 1.63 for the rotarod test, 0.71 for the cylinder test, and 1.11 for infarct volume reduction). With further analysis, it was found that the administration time poststroke, NSPC donor species, and transplantation immunogenicity had close correlations with the degree of infarct volume reduction. The NSPC dosage delivered into the brain parenchyma was also negatively correlated with the effect of the cylinder test. Intriguingly, endogenous apoptosis inhibition and axonal regeneration played the most critical role in intraparenchymal NSPC transplantation among the cellular mechanisms. These results indicate that intraparenchymal NSPC transplantation is beneficial for neurobehavioral and histological improvement and is relatively safe for ischemic stroke animals. Therefore, intraparenchymal NSPC transplantation is a promising treatment for stroke patients.
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DOI：
10.1136/bmj.b2535
发表时间：
2009-07-21
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Moher D;Liberati A;Tetzlaff J;Altman DG;PRISMA Group
通讯作者：
PRISMA Group
影响因子：
4.6
作者：
Chen L;Zhang G;Gu Y;Guo X
通讯作者：
Guo X
影响因子：
2.9
作者：
Chen, Lukui;Qiu, Rong;Gu, Ning
通讯作者：
Gu, Ning
影响因子：
5.1
作者：
Hou, Boru;Ma, Junning;Ren, Haijun
通讯作者：
Ren, Haijun
影响因子：
6
作者：
Hesse, S;Werner, C
通讯作者：
Werner, C