Adapting Human-Based Transcutaneous Spinal Cord Stimulation to Develop a Clinically Relevant Animal Model.
Adapting Human-Based Transcutaneous Spinal Cord Stimulation to Develop a Clinically Relevant Animal Model.
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调整基于人类的经皮脊髓刺激以开发与临床相关的动物模型。
DOI：
10.3390/jcm11072023
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发表时间：
2022-04-05
影响因子：
3.9
通讯作者：
Côté MP
中科院分区：
文献类型：
--
作者：
Malloy DC;Knikou M;Côté MP
经皮脊髓刺激（tSCS）作为一种神经调节策略，作为促进脊髓损伤（SCI）后功能恢复的方法受到了广泛关注。然而，由于tSCS的非侵入性，研究主要集中在人类应用上。这就迫切需要开发一种合适的动物模型，以进一步了解这种治疗干预在功能和神经解剖可塑性方面的作用，并优化刺激方案。本研究的目的是建立一种新的胸腰椎tSCS动物模型，(1)可以准确地概括健康人体的研究，(2)可以在脊髓损伤后以最小的约束接受重复和稳定的tSCS治疗，同时电极保持一致的位置。我们表明，我们的模型显示了双侧多节段腿部肌肉的诱发电位，与人类的特征相当。我们的数据还表明，tSCS像人类一样主要激活背根结构，从而解释了两个物种之间不同的电极与身体大小比率。最后，在完全脊髓横断后的清醒大鼠中重复tSCS治疗方案是可行的、可耐受的和安全的，甚至需要最小的身体约束。此外，重复tSCS能够调节脊髓损伤后的运动输出，为进一步研究基于刺激的神经可塑性和优化治疗提供了途径。
Transcutaneous spinal cord stimulation (tSCS) as a neuromodulatory strategy has received great attention as a method to promote functional recovery after spinal cord injury (SCI). However, due to the noninvasive nature of tSCS, investigations have primarily focused on human applications. This leaves a critical need for the development of a suitable animal model to further our understanding of this therapeutic intervention in terms of functional and neuroanatomical plasticity and to optimize stimulation protocols. The objective of this study is to establish a new animal model of thoracolumbar tSCS that (1) can accurately recapitulate studies in healthy humans and (2) can receive a repeated and stable tSCS treatment after SCI with minimal restraint, while the electrode remains consistently positioned. We show that our model displays bilateral evoked potentials in multisegmental leg muscles characteristically comparable to humans. Our data also suggest that tSCS mainly activates dorsal root structures like in humans, thereby accounting for the different electrode-to-body-size ratio between the two species. Finally, a repeated tSCS treatment protocol in the awake rat after a complete spinal cord transection is feasible, tolerable, and safe, even with minimal body restraint. Additionally, repeated tSCS was capable of modulating motor output after SCI, providing an avenue to further investigate stimulation-based neuroplasticity and optimize treatment.
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影响因子：
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